TY - JOUR
T1 - Impaired host defense in mice lacking ONZIN
AU - Ledford, Julie G.
AU - Kovarova, Marina
AU - Koller, Beverly H.
PY - 2007/4/15
Y1 - 2007/4/15
N2 - ONZIN is a small, cysteine-rich-peptide of unique structure that is conserved in all vertebrates examined to date. We show that ONZIN is expressed at high levels in epithelial cells of the intestinal tract, the lung, and in cells of the immune system including macrophages and granulocytes. Because this pattern of expression is suggestive of a role in innate immune function, we have generated mice lacking this protein and examined their ability to respond to challenge with infectious agents. Onzin-/- mice show a heightened innate immune response after induction of acute peritonitis with Klebsiella pneumoniae. This increased response is consistent with an increased bacterial burden in the Onzin-/- mice. Ex vivo studies show that, whereas phagocytosis is not altered in Onzin-/- neutrophils, phagocytes lacking this protein kill bacteria less effectively. This result identifies ONZIN as a novel class of intracelular protein required for optimal function of the neutrophils after uptake of bacteria.
AB - ONZIN is a small, cysteine-rich-peptide of unique structure that is conserved in all vertebrates examined to date. We show that ONZIN is expressed at high levels in epithelial cells of the intestinal tract, the lung, and in cells of the immune system including macrophages and granulocytes. Because this pattern of expression is suggestive of a role in innate immune function, we have generated mice lacking this protein and examined their ability to respond to challenge with infectious agents. Onzin-/- mice show a heightened innate immune response after induction of acute peritonitis with Klebsiella pneumoniae. This increased response is consistent with an increased bacterial burden in the Onzin-/- mice. Ex vivo studies show that, whereas phagocytosis is not altered in Onzin-/- neutrophils, phagocytes lacking this protein kill bacteria less effectively. This result identifies ONZIN as a novel class of intracelular protein required for optimal function of the neutrophils after uptake of bacteria.
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U2 - 10.4049/jimmunol.178.8.5132
DO - 10.4049/jimmunol.178.8.5132
M3 - Article
C2 - 17404296
AN - SCOPUS:34247127884
SN - 0022-1767
VL - 178
SP - 5132
EP - 5143
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -