Impaired bone anabolic response to parathyroid hormone in Fgf2-/- and Fgf2+/- mice

M. M. Hurley; Y. Okada; L. Xiao; Y. Tanaka; M. Ito; N. Okimoto; T. Nakamura; C. J. Rosen; T. Doetschman; J. D. Coffin

doi:10.1016/j.bbrc.2006.01.044

Impaired bone anabolic response to parathyroid hormone in Fgf2-/- and Fgf2+/- mice

M. M. Hurley, Y. Okada, L. Xiao, Y. Tanaka, M. Ito, N. Okimoto, T. Nakamura, C. J. Rosen, T. Doetschman, J. D. Coffin

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

Since parathyroid hormone (PTH) increased FGF2 mRNA and protein expression in osteoblasts, and serum FGF-2 was increased in osteoporotic patients treated with PTH, we assessed whether the anabolic effect of PTH was impaired in Fgf2-/- mice. Eight-week-old Fgf2+/+ and Fgf2-/- male mice were treated with rhPTH 1-34 (80 μg/kg) for 4 weeks. Micro-CT and histomorphometry demonstrated that PTH significantly increased parameters of bone formation in femurs from Fgf2+/+ mice but the changes were smaller and not significant in Fgf2-/- mice. IGF-1 was significantly reduced in serum from PTH-treated Fgf2-/- mice. DEXA analysis of femurs from Fgf2+/+, Fgf2+/-, and Fgf2-/- mice treated with rhPTH (160 μg/kg) for 10 days showed that PTH significantly increased femoral BMD in Fgf2+/+ by 18%; by only 3% in Fgf2+/- mice and reduced by 3% in Fgf2-/- mice. We conclude that endogenous Fgf2 is important for maximum bone anabolic effect of PTH in mice.

Original language	English (US)
Pages (from-to)	989-994
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	341
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2006.01.044
State	Published - Mar 24 2006
Externally published	Yes

Keywords

Bone formation
DEXA
Fgf2 haploinsufficient
Fgf2 null
Mice
Micro-CT
PTH

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2006.01.044

Cite this

@article{9de4b6405a91443f9dd9ddebd5524439,

title = "Impaired bone anabolic response to parathyroid hormone in Fgf2-/- and Fgf2+/- mice",

abstract = "Since parathyroid hormone (PTH) increased FGF2 mRNA and protein expression in osteoblasts, and serum FGF-2 was increased in osteoporotic patients treated with PTH, we assessed whether the anabolic effect of PTH was impaired in Fgf2-/- mice. Eight-week-old Fgf2+/+ and Fgf2-/- male mice were treated with rhPTH 1-34 (80 μg/kg) for 4 weeks. Micro-CT and histomorphometry demonstrated that PTH significantly increased parameters of bone formation in femurs from Fgf2+/+ mice but the changes were smaller and not significant in Fgf2-/- mice. IGF-1 was significantly reduced in serum from PTH-treated Fgf2-/- mice. DEXA analysis of femurs from Fgf2+/+, Fgf2+/-, and Fgf2-/- mice treated with rhPTH (160 μg/kg) for 10 days showed that PTH significantly increased femoral BMD in Fgf2+/+ by 18%; by only 3% in Fgf2+/- mice and reduced by 3% in Fgf2-/- mice. We conclude that endogenous Fgf2 is important for maximum bone anabolic effect of PTH in mice.",

keywords = "Bone formation, DEXA, Fgf2 haploinsufficient, Fgf2 null, Mice, Micro-CT, PTH",

author = "Hurley, {M. M.} and Y. Okada and L. Xiao and Y. Tanaka and M. Ito and N. Okimoto and T. Nakamura and Rosen, {C. J.} and T. Doetschman and Coffin, {J. D.}",

note = "Funding Information: This work is supported in part by NIH Grant AG021189 to M.M. Hurley.",

year = "2006",

month = mar,

day = "24",

doi = "10.1016/j.bbrc.2006.01.044",

language = "English (US)",

volume = "341",

pages = "989--994",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "4",

}

TY - JOUR

T1 - Impaired bone anabolic response to parathyroid hormone in Fgf2-/- and Fgf2+/- mice

AU - Hurley, M. M.

AU - Okada, Y.

AU - Xiao, L.

AU - Tanaka, Y.

AU - Ito, M.

AU - Okimoto, N.

AU - Nakamura, T.

AU - Rosen, C. J.

AU - Doetschman, T.

AU - Coffin, J. D.

N1 - Funding Information: This work is supported in part by NIH Grant AG021189 to M.M. Hurley.

PY - 2006/3/24

Y1 - 2006/3/24

N2 - Since parathyroid hormone (PTH) increased FGF2 mRNA and protein expression in osteoblasts, and serum FGF-2 was increased in osteoporotic patients treated with PTH, we assessed whether the anabolic effect of PTH was impaired in Fgf2-/- mice. Eight-week-old Fgf2+/+ and Fgf2-/- male mice were treated with rhPTH 1-34 (80 μg/kg) for 4 weeks. Micro-CT and histomorphometry demonstrated that PTH significantly increased parameters of bone formation in femurs from Fgf2+/+ mice but the changes were smaller and not significant in Fgf2-/- mice. IGF-1 was significantly reduced in serum from PTH-treated Fgf2-/- mice. DEXA analysis of femurs from Fgf2+/+, Fgf2+/-, and Fgf2-/- mice treated with rhPTH (160 μg/kg) for 10 days showed that PTH significantly increased femoral BMD in Fgf2+/+ by 18%; by only 3% in Fgf2+/- mice and reduced by 3% in Fgf2-/- mice. We conclude that endogenous Fgf2 is important for maximum bone anabolic effect of PTH in mice.

AB - Since parathyroid hormone (PTH) increased FGF2 mRNA and protein expression in osteoblasts, and serum FGF-2 was increased in osteoporotic patients treated with PTH, we assessed whether the anabolic effect of PTH was impaired in Fgf2-/- mice. Eight-week-old Fgf2+/+ and Fgf2-/- male mice were treated with rhPTH 1-34 (80 μg/kg) for 4 weeks. Micro-CT and histomorphometry demonstrated that PTH significantly increased parameters of bone formation in femurs from Fgf2+/+ mice but the changes were smaller and not significant in Fgf2-/- mice. IGF-1 was significantly reduced in serum from PTH-treated Fgf2-/- mice. DEXA analysis of femurs from Fgf2+/+, Fgf2+/-, and Fgf2-/- mice treated with rhPTH (160 μg/kg) for 10 days showed that PTH significantly increased femoral BMD in Fgf2+/+ by 18%; by only 3% in Fgf2+/- mice and reduced by 3% in Fgf2-/- mice. We conclude that endogenous Fgf2 is important for maximum bone anabolic effect of PTH in mice.

KW - Bone formation

KW - DEXA

KW - Fgf2 haploinsufficient

KW - Fgf2 null

KW - Mice

KW - Micro-CT

KW - PTH

UR - http://www.scopus.com/inward/record.url?scp=32344440164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32344440164&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.01.044

DO - 10.1016/j.bbrc.2006.01.044

M3 - Article

C2 - 16455048

AN - SCOPUS:32344440164

SN - 0006-291X

VL - 341

SP - 989

EP - 994

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Impaired bone anabolic response to parathyroid hormone in Fgf2-/- and Fgf2+/- mice

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this