@article{a0a9136f02ad43a0b350de2153910202,
title = "Immunopathologic studies of experimental pemphigus-like autoantibodies and bullous-like lesions.",
author = "Ablin, \{R. J.\}",
note = "Funding Information: bullous skin diseases of man have been the sub- taining antigen(s) possessing both tissue and ject of numerous investigations. A comprehen- serologic specificity for autoantibodies to the sive review summarizing these investigations IC substance of stratified squamous epithelium has recently appeared (1). These studies have have been developed (9—11). Further efforts focused primarily on the possible diagnostic were concentrated on the ethanol soluble frac-and prognostic value of employing the adjunct tion as preliminary studies (12) indicated that sensitivity and specificity of immunologic reac- the aqueous fractions contained numerous tions, i.e., immunofluorescence (IF), to the es- serum proteins in addition to the IC anti- tablished histopathologiccharacteristics of gen (s). these dermatoses as an aid in differentiating Heteroimmunization of rabbits with an pemphigus and pemphigoid from other bullous ethanolic extract of bovine esophageal mucosa diseases and from each other (2—4). incorporating Freund adjuvant resulted in the As previous studies (3, 5—8) have demon- formation of pemphigus-like antibodies yield- strated that there appears to be a direct rela- ing indirect IF staining patterns of the IC areas tionship between the severity of pemphigus and of stratified squamous epithelium indistinguish- the titer of intercellular (IC) antibodies, the able from those of human pemphigus autoan-question arose as to whether or not the char- tibodies (10, 11, 13). Initially, these appeared acteristic histopathologic changes in pemphigus as heteroantibodies, i.e., they reacted with only might be attributed in part to immunologic bovine epithelium, however, upon continued phenomena. immunization, antibodies reactive with rabbit Suitable means for the preparation of aque-epithelium appeared (13). Pemphigus-like an- ous and ethanolic extracts of human, rhesus tibodies were shown to be autoantibodies by the demonstration of positive in vitro reactions with the antibody-producer's own tissues (13). These antibodies were shown to be tissue specific, and histologically and serologically in- distinguishable from the IC autoantibodies of pemphigus. Similar studies have been reported on the isolation and antigenicity of an {"}insoluble{"} Received July 24, 1969; accepted for publication August 13, 1969. Supported in part by a grant from the John A. Hartford Foundation, Inc. and by USPHS Grants AM 10537 and 2 Ti AI-130-08. Presented in part at the Thirtieth Annual Meeting of the Society for Investigative Derma-tology, Inc., July 12—13, 1969, New York, N.Y. * From the Division of Immunology, Millard Fillmore Hospital, Buffalo, New York 14209.",
year = "1969",
month = dec,
doi = "10.1038/jid.1969.177",
language = "English (US)",
volume = "53",
pages = "463--469",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Elsevier B.V.",
number = "6",
}