Immunological characterization of bronchoalveolar lavage fluid in patients with acute pulmonary coccidioidomycosis

Lance A. Nesbit, Kenneth S. Knox, Chinh T. Nguyen, Justin Roesch, L. Joseph Wheat, Suzanne M. Johnson, Demosthenes Pappagianis, Suzette Chavez, Neil M. Ampel

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Background. The specific cellular immunological characteristics of bronchoalveolar lavage (BAL) fluid in acute pulmonary coccidioidomycosis have not been defined.Methods. BAL fluid from patients living in a coccidioidomycosis-endemic region of Arizona who were undergoing bronchoscopy because of pulmonary infiltrates was analyzed. Mononuclear cells from BAL fluid and peripheral blood mononuclear cells (PBMCs) were incubated with the coccidioidal antigen T27K in vitro, and cellular immunological assays were performed.Results. Forty-six patients were studied. Twelve received a diagnosis of acute pulmonary coccidioidomycosis, 17 received other diagnoses, and 17 had no diagnosis established. There was an increased proportion of polyfunctional CD8+ T cells after antigen stimulation from subjects with coccidioidomycosis as compared to those with another diagnosis (P =. 025). In cells collected from BAL fluid and in PBMCs, the concentrations of interferon, tumor necrosis factor α, and interleukin 17 (IL-17) were all significantly increased in samples from those with acute pulmonary coccidioidomycosis, compared with the other 2 groups (for all, P<.05).Conclusions. When incubated in vitro with a coccidioidal antigen preparation, cells from both BAL fluid and peripheral blood obtained from patients with pulmonary coccidioidomycosis demonstrated specific cellular immune responses, including expression of IL-17.

Original languageEnglish (US)
Pages (from-to)857-863
Number of pages7
JournalJournal of Infectious Diseases
Issue number5
StatePublished - Sep 1 2013


  • bronchoalveolar lavage
  • cellular immunity
  • coccidioidomycosis
  • human

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases


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