Immunohistochemical validation of a novel epithelial and a novel stromal marker of pancreatic ductal adenocarcinoma identified by global expression microarrays: Sea urchin fascin homolog and heat shock protein 47

Anirban Maitra, Christine Iacobuzio-Donahue, Ayman Rahman, Taylor A. Sohn, Peter Argani, Renee Meyer, Charles J. Yeo, John L. Cameron, Michael Goggins, Scott E. Kern, Raheela Ashfaq, Ralph H. Hruban, Robb E. Wilentz

Research output: Contribution to journalArticlepeer-review

119 Scopus citations

Abstract

We extended the results of a previous microarray analysis by immunohistochemical validation of differential protein expression in a series of 57 surgically resected infiltrating ductal pancreatic adenocarcinomas. Two representative genes were examined: sea urchin fascin homolog (overexpressed in both cell lines and primary tumors) and heat shock protein 47 (HSP47; overexpressed in primary tumors only). Protein expression also was evaluated in the precursor lesions of pancreatic cancer, pancreatic intraepithelial neoplasia (PanIN), and normal ductal epithelium. Fascin expression was seen in the neoplastic cells of 54 (95%) of 57 ductal adenocarcinomas but not in 49 (94%) of 52 adjacent nonneoplastic epithelium. In the multistep pathogenesis of ductal adenocarcinomas, fascin expression seemed to be a late event, usually present in PanINs 2 and 3. HSP47 expression was almost universal and most intense in the ductal adenocarcinoma-associated stromal desmoplasia (57/57, although 37 cases (65%) also expressed HSP47 in the neoplastic epithelium. HSP47 expression was absent in the majority of nonneoplastic pancreata (46 [88%]). Fascin and HSP47 are novel tumor markers with potential diagnostic and therapeutic implications for pancreatic carcinoma. These results establish the usefulness of global expression platforms to identify novel tumor markers.

Original languageEnglish (US)
Pages (from-to)52-59
Number of pages8
JournalAmerican journal of clinical pathology
Volume118
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Heat shock protein 47
  • Immunohistochemistry
  • Microarray
  • Pancreas
  • Sea urchin fascin homolog

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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