TY - JOUR
T1 - Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods
AU - Nagle, R. B.
AU - Clark, V. A.
AU - McDaniel, K. M.
AU - Davis, J. R.
PY - 1983
Y1 - 1983
N2 - A comparison of five immunohistochemical methods for the demonstration of keratins in humans ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase-antiperoxidase (PAP), indirect peroxidase, or the avidin-biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.
AB - A comparison of five immunohistochemical methods for the demonstration of keratins in humans ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase-antiperoxidase (PAP), indirect peroxidase, or the avidin-biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.
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U2 - 10.1177/31.8.6190854
DO - 10.1177/31.8.6190854
M3 - Article
C2 - 6190854
AN - SCOPUS:0020613349
SN - 0022-1554
VL - 31
SP - 1010
EP - 1014
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
IS - 8
ER -