Immunohistochemical analysis of receptor tyrosine kinase signal transduction activity in chordoma

J. H. Fasig, W. D. Dupont, B. J. Lafleur, S. J. Olson, J. M.M. Cates

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Aims: Currently, there are no effective chemotherapeutic protocols for chordoma. Reports of receptor tyrosine kinase (RTK) expression in chordoma suggest that these tumours may respond to kinase inhibitor therapy. However, RTK signalling activity has not been extensively investigated in chordoma. Methods: A tissue microarray containing 21 cases of chordoma was analysed for expression of a number of proteins involved in signal transduction from RTKs by immunohistochemistry. Results: Platelet-derived growth factor receptor-β, epidermal growth factor receptor (EGFR), KIT and HER2 were detected in 100%, 67%, 33% and 0% of cases, respectively. Platelet-derived growth factor receptor-β staining was of moderate-to-strong intensity in 20 of 21 cases. In contrast, KIT immunoreactivity was weak and focal in each of the seven positive cases. Total EGFR staining was variable; weak staining for phosphorylated EGFR was detected in nine cases. Phosphorylated isoforms of p44/42 mitogen-activated protein kinase, Akt and STAT3, indicative of tyrosine kinase activity, were detected in 86%, 76% and 67% of cases, respectively. Conclusions: Chordomas commonly express RTKs and activated signal transduction molecules. Although there were no statistically significant correlations between the expression of any of the markers studied and disease-free survival or tumour location, the results nonetheless indicate that chordomas may respond to RTK inhibitors or modulators of other downstream signalling molecules.

Original languageEnglish (US)
Pages (from-to)95-104
Number of pages10
JournalNeuropathology and Applied Neurobiology
Volume34
Issue number1
DOIs
StatePublished - Feb 2008
Externally publishedYes

Keywords

  • Chordoma
  • EGFR
  • HER2
  • PDGFR
  • Receptor tyrosine kinase
  • Signal transduction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Neurology
  • Clinical Neurology
  • Physiology (medical)

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