Immunohistochemical analysis of cathepsin D in prostate carcinoma.

R. Makar, A. Mason, J. M. Kittelson, G. T. Bowden, A. E. Cress, R. B. Nagle

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22 Scopus citations


Cathepsin D is a carboxyl protease that has been implicated as an important factor in tumor cell invasion. Sixty-nine cases of primary adenocarcinoma of the prostate were studied by the indirect immunoperoxidase method using a primary monoclonal anti-cathepsin D antibody. Immunoreactivity was graded from 0 (negative) to 4+ (intense reaction). The normal tubuloalveolar glands were, in general, negative. However, nine cases revealed focal staining of nonneoplastic luminal cells. Basal cells were negative except in areas of basal-cell hyperplasia, which were intensely positive. Thirty-nine of 78 carcinoma samples revealed 2+ or greater positive punctate lysosomal staining. In the 39 positive-stained cases, the reactivity was diffuse in three and focal in the remainder. The percentage of carcinoma cases whose worst lesions stained 2+ or greater showed a nonsignificant (P = 0.055) relation to Gleason grade but a significant (P = 0.031) relationship to pathologic stage. Thus, cathepsin D may prove to be a useful marker of prostate cancer progression.

Original languageEnglish (US)
Pages (from-to)747-751
Number of pages5
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Issue number7
StatePublished - Sep 1994

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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