TY - JOUR
T1 - Immunoactive atrial natriuretic peptide in the rat eye
T2 - Molecular forms in anterior uvea and retina
AU - Stone, Richard A.
AU - Glembotski, Christopher C.
N1 - Funding Information:
ACKNOWLEDGMENTS: We thank Ms. Cheryl M. Wilson for excellent technical assistance. Supported by NIH grants EY-05454, NS-20396, B.R.S.G. SO7-RR-05415-23, an unrestricted grant from Allergan Pharmaceuticals, Grant-in-aid 84 653 from the American Heart Association with funds contributed in part by the Philadelphia Chapter of the American Heart Association, and the Edwin M. Lavino Trust of the Scheie Eye Institute.
PY - 1986/1/29
Y1 - 1986/1/29
N2 - Acid extracts of rat anterior uvea and retina contain immunoactive atrial natriuretic peptide. Gel filtration chromatography demonstrates that the major forms of the uveal and retinal material have approximate molecular weights of 2400 and 1750 daltons respectively, similar in size to the hypothalamic form of the peptide but clearly distinguishable from the larger, cardiac form. When further analyzed by RP-HPLC, the immunoactive material from both ocular tissues elutes at a position similar to atrial natriuretic peptide-28, again distinguishing the ocular and cardiac forms of immunoactive material. These results suggest potential roles for atrial natriuretic peptide both in ocular vegetative physiology and in retinal function.
AB - Acid extracts of rat anterior uvea and retina contain immunoactive atrial natriuretic peptide. Gel filtration chromatography demonstrates that the major forms of the uveal and retinal material have approximate molecular weights of 2400 and 1750 daltons respectively, similar in size to the hypothalamic form of the peptide but clearly distinguishable from the larger, cardiac form. When further analyzed by RP-HPLC, the immunoactive material from both ocular tissues elutes at a position similar to atrial natriuretic peptide-28, again distinguishing the ocular and cardiac forms of immunoactive material. These results suggest potential roles for atrial natriuretic peptide both in ocular vegetative physiology and in retinal function.
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U2 - 10.1016/S0006-291X(86)80523-X
DO - 10.1016/S0006-291X(86)80523-X
M3 - Article
C2 - 2936342
AN - SCOPUS:0022544374
SN - 0006-291X
VL - 134
SP - 1022
EP - 1028
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -