Immuno-gene therapy of melanoma by tumor antigen epitope modified IFN-γ

Xianghui He; Phoebe Luo; Tom C. Tsang; Tong Zhang; David T. Harris

doi:10.1007/s00262-004-0634-5

Immuno-gene therapy of melanoma by tumor antigen epitope modified IFN-γ

Xianghui He, Phoebe Luo, Tom C. Tsang, Tong Zhang, David T. Harris

Immunobiology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Cytokine-based vaccines play a major part in tumor immuno-gene therapy. However, down-regulated antigen expression on tumor cells may diminish the immuno-potentiating aspects of cellular vaccines. In this study, we coexpressed a tumor antigen epitope with IFN-γ in the same gene by replacing the IFN-γ signal peptide with an antigen epitope-expressing signal peptide. We then investigated the effect of the antigen epitope-incorporated IFN-γ on the immunotherapy of murine melanoma B16 tumors. Results showed that TRP-2 epitope-expressing IFN-γ decreased B16 tumorigenicity and enhanced its immunogenicity after gene transfer. Protective immunity against wild type B16 tumors was induced by vaccination with IFN-γ transiently gene-modified tumor cells. These data suggest that cellular vaccines engineered to express an antigen epitope within an immunostimulatory cytokine could potentiate the immunization effect.

Original language	English (US)
Pages (from-to)	741-749
Number of pages	9
Journal	Cancer Immunology, Immunotherapy
Volume	54
Issue number	8
DOIs	https://doi.org/10.1007/s00262-004-0634-5
State	Published - Jul 2005

Keywords

Antigen epitope
Cytokine
Immunotherapy
Tumor
Vaccine

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Oncology
Cancer Research

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10.1007/s00262-004-0634-5

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abstract = "Cytokine-based vaccines play a major part in tumor immuno-gene therapy. However, down-regulated antigen expression on tumor cells may diminish the immuno-potentiating aspects of cellular vaccines. In this study, we coexpressed a tumor antigen epitope with IFN-γ in the same gene by replacing the IFN-γ signal peptide with an antigen epitope-expressing signal peptide. We then investigated the effect of the antigen epitope-incorporated IFN-γ on the immunotherapy of murine melanoma B16 tumors. Results showed that TRP-2 epitope-expressing IFN-γ decreased B16 tumorigenicity and enhanced its immunogenicity after gene transfer. Protective immunity against wild type B16 tumors was induced by vaccination with IFN-γ transiently gene-modified tumor cells. These data suggest that cellular vaccines engineered to express an antigen epitope within an immunostimulatory cytokine could potentiate the immunization effect.",

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AU - He, Xianghui

AU - Luo, Phoebe

AU - Tsang, Tom C.

AU - Zhang, Tong

AU - Harris, David T.

PY - 2005/7

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N2 - Cytokine-based vaccines play a major part in tumor immuno-gene therapy. However, down-regulated antigen expression on tumor cells may diminish the immuno-potentiating aspects of cellular vaccines. In this study, we coexpressed a tumor antigen epitope with IFN-γ in the same gene by replacing the IFN-γ signal peptide with an antigen epitope-expressing signal peptide. We then investigated the effect of the antigen epitope-incorporated IFN-γ on the immunotherapy of murine melanoma B16 tumors. Results showed that TRP-2 epitope-expressing IFN-γ decreased B16 tumorigenicity and enhanced its immunogenicity after gene transfer. Protective immunity against wild type B16 tumors was induced by vaccination with IFN-γ transiently gene-modified tumor cells. These data suggest that cellular vaccines engineered to express an antigen epitope within an immunostimulatory cytokine could potentiate the immunization effect.

AB - Cytokine-based vaccines play a major part in tumor immuno-gene therapy. However, down-regulated antigen expression on tumor cells may diminish the immuno-potentiating aspects of cellular vaccines. In this study, we coexpressed a tumor antigen epitope with IFN-γ in the same gene by replacing the IFN-γ signal peptide with an antigen epitope-expressing signal peptide. We then investigated the effect of the antigen epitope-incorporated IFN-γ on the immunotherapy of murine melanoma B16 tumors. Results showed that TRP-2 epitope-expressing IFN-γ decreased B16 tumorigenicity and enhanced its immunogenicity after gene transfer. Protective immunity against wild type B16 tumors was induced by vaccination with IFN-γ transiently gene-modified tumor cells. These data suggest that cellular vaccines engineered to express an antigen epitope within an immunostimulatory cytokine could potentiate the immunization effect.

KW - Antigen epitope

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KW - Immunotherapy

KW - Tumor

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Immuno-gene therapy of melanoma by tumor antigen epitope modified IFN-γ

Abstract

Keywords

ASJC Scopus subject areas

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