@article{0c5eb9ed9e5642ffb51b8f6eccf66cdb,
title = "Immune Monitoring Reveals Fusion Peptide Priming to Imprint Cross-Clade HIV-Neutralizing Responses with a Characteristic Early B Cell Signature",
abstract = "Immune monitoring of B cells in response to vaccination can enable early insights, even in the absence of serum neutralization. Cheng et al. observe an early B cell signature in NHPs predictive of the vaccine outcome, with priming of HIV FP imprinting cross-clade neutralizing FP-directed vaccine responses.",
keywords = "HIV vaccine, NHP, early signature, envelope trimer, fusion peptide, immune monitoring, immunization regimen, immunogen cocktail, imprinting, prime-boost immunization",
author = "{VRC Production Program} and Cheng Cheng and Hongying Duan and Kai Xu and Chuang, {Gwo Yu} and Corrigan, {Angela R.} and Hui Geng and Sijy O'Dell and Li Ou and Michael Chambers and Anita Changela and Xuejun Chen and Foulds, {Kathryn E.} and Sarfo, {Edward K.} and Jafari, {Alexander J.} and Hill, {Kurt R.} and Rui Kong and Kevin Liu and Todd, {John P.} and Yaroslav Tsybovsky and Raffaello Verardi and Shuishu Wang and Yiran Wang and Winston Wu and Tongqing Zhou and Arnold, {Frank J.} and Doria-Rose, {Nicole A.} and Koup, {Richard A.} and McDermott, {Adrian B.} and Scorpio, {Diana G.} and Michael Worobey and Lawrence Shapiro and Mascola, {John R.} and Kwong, {Peter D.}",
note = "Funding Information: We thank J. Hill, E. Smit, R. Nguyen, D. Ambrozak, and S.P. Perfetto of the Flow Cytometry Core at the Vaccine Research Center (VRC) for assistance with B cell sorting; J. Noor and E. McCarthy of the Translational Research Program and Bioqual veterinary technical staff; K. Saunders and B.F. Haynes for discussions on CH505; J. Stuckey for assistance with figures; members of the Non-human Primate Immunogenicity Core at the VRC for assistance with the NHP studies; and members of the Virology Laboratory and Vector Core, VRC, for discussions and comments on the manuscript. Support for this work was provided by the Intramural Research Program of the Vaccine Research Center , National Institute of Allergy and Infectious Diseases ( NIAID ), by federal funds from the Frederick National Laboratory for Cancer Research, NIH , under Contract HHSN261200800001 , and by the International AIDS Vaccine Initiative{\textquoteright}s ( IAVI {\textquoteright}s) Neutralizing Antibody Consortium. M.W. was supported by a grant from the David and Lucile Packard Foundation . Funding Information: We thank J. Hill, E. Smit, R. Nguyen, D. Ambrozak, and S.P. Perfetto of the Flow Cytometry Core at the Vaccine Research Center (VRC) for assistance with B cell sorting; J. Noor and E. McCarthy of the Translational Research Program and Bioqual veterinary technical staff; K. Saunders and B.F. Haynes for discussions on CH505; J. Stuckey for assistance with figures; members of the Non-human Primate Immunogenicity Core at the VRC for assistance with the NHP studies; and members of the Virology Laboratory and Vector Core, VRC, for discussions and comments on the manuscript. Support for this work was provided by the Intramural Research Program of the Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), by federal funds from the Frederick National Laboratory for Cancer Research, NIH, under Contract HHSN261200800001, and by the International AIDS Vaccine Initiative's (IAVI's) Neutralizing Antibody Consortium. M.W. was supported by a grant from the David and Lucile Packard Foundation. C.C. and K.X. co-headed NHP immunizations; C.C. headed ELISA readout; K.X. developed CH505 trimers; H.D. and A.R.C. performed B cell analysis and statistical analysis; C.C. H.D. and K.X. co-drafted the manuscript and prepared figures; G.-Y.C. and Y.W. performed statistical analysis; A.R.C. A.J.J. K.R.H. and E.K.S. performed plasma ELISAs; H.G. T.Z. K.L. and V.P.P. prepared trimer immunogens; S.O.D. and N.A.D.-R. performed neutralization; L.O. prepared FP immunogens; M.C. analyzed antigenic properties of trimer proteins using Meso Scale Discovery (MSD); A.C. coordinated access for BG505 trimer; XC co-provided trimer probe for FACS; KEF led the NHP core for sample processing; R.K. and W.W. performed FP competition neutralization; J.P.T. coordinated NHP studies; Y.T. performed negative-strain EM; R.V. provided cell lines for protein production; S.W. assisted with manuscript assembly; F.A. headed V.P.P. producing BG505 immunogen; R.A.K. contributed analysis related to immune system parameters and outcomes; A.B.M. headed antigenic analysis by MSD; D.G.S. headed the animal facility; M.W. contributed analysis and insight related to imprinting; and L.S. J.R.M. and P.D.K. headed the study and co-wrote the manuscript with all authors providing comments and revisions. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020",
year = "2020",
month = aug,
day = "4",
doi = "10.1016/j.celrep.2020.107981",
language = "English (US)",
volume = "32",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",
}