Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea)

Michael J. Danilich; Kandice Kottke-Marchant; Dominic Gervasio; Tracey L. Bonfield; James M. Anderson; Roger E. Marchant

Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea)

Michael J. Danilich, Kandice Kottke-Marchant, Dominic Gervasio, Tracey L. Bonfield, James M. Anderson, Roger E. Marchant

Research output: Contribution to conference › Paper › peer-review

Abstract

In this paper, the combination of the excellent bulk properties of a segmented polyurethane and the excellent surface properties of a RF plasma polymer for the immobilization of a bioactive molecule is discussed. Specifically, glucose oxidase (GOx) has been covalently immobilized to poly(etherurethaneurea) (PEUU) films which were first modified with RF plasma polymerized N-vinyl-2-pyrrolidone (PPNVP). The results of the RIA and the immunochemical stain indicated that washes based on published procedures were inadequate in removing physically adsorbed GOx from the surfaces of PEUU and PPNVP/PEUU controls. The only wash which was effective consisted of continuous magnetic stirring in 900 ml of 2% sodium dodecyl sulfate, 2% Triton X-100, and 20 mM sodium phosphate (pH 7.0) for 20-24 hrs. each at 5°C.

Original language	English (US)
Pages	132
Number of pages	1
State	Published - 1991
Externally published	Yes
Event	17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium - Scottsdale, AZ, USA Duration: May 1 1991 → May 5 1991

Other

Other	17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium
City	Scottsdale, AZ, USA
Period	5/1/91 → 5/5/91

ASJC Scopus subject areas

General Materials Science

Cite this

Danilich, M. J., Kottke-Marchant, K., Gervasio, D., Bonfield, T. L., Anderson, J. M., & Marchant, R. E. (1991). Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea). 132. Paper presented at 17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium, Scottsdale, AZ, USA.

Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea). / Danilich, Michael J.; Kottke-Marchant, Kandice; Gervasio, Dominic et al.
1991. 132 Paper presented at 17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium, Scottsdale, AZ, USA.

Research output: Contribution to conference › Paper › peer-review

Danilich, MJ, Kottke-Marchant, K, Gervasio, D, Bonfield, TL, Anderson, JM & Marchant, RE 1991, 'Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea)', Paper presented at 17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium, Scottsdale, AZ, USA, 5/1/91 - 5/5/91 pp. 132.

@conference{81e38ad0b31143119d873a1d68c83647,

title = "Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea)",

abstract = "In this paper, the combination of the excellent bulk properties of a segmented polyurethane and the excellent surface properties of a RF plasma polymer for the immobilization of a bioactive molecule is discussed. Specifically, glucose oxidase (GOx) has been covalently immobilized to poly(etherurethaneurea) (PEUU) films which were first modified with RF plasma polymerized N-vinyl-2-pyrrolidone (PPNVP). The results of the RIA and the immunochemical stain indicated that washes based on published procedures were inadequate in removing physically adsorbed GOx from the surfaces of PEUU and PPNVP/PEUU controls. The only wash which was effective consisted of continuous magnetic stirring in 900 ml of 2% sodium dodecyl sulfate, 2% Triton X-100, and 20 mM sodium phosphate (pH 7.0) for 20-24 hrs. each at 5°C.",

author = "Danilich, {Michael J.} and Kandice Kottke-Marchant and Dominic Gervasio and Bonfield, {Tracey L.} and Anderson, {James M.} and Marchant, {Roger E.}",

year = "1991",

language = "English (US)",

pages = "132",

note = "17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium ; Conference date: 01-05-1991 Through 05-05-1991",

}

TY - CONF

T1 - Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea)

AU - Danilich, Michael J.

AU - Kottke-Marchant, Kandice

AU - Gervasio, Dominic

AU - Bonfield, Tracey L.

AU - Anderson, James M.

AU - Marchant, Roger E.

PY - 1991

Y1 - 1991

N2 - In this paper, the combination of the excellent bulk properties of a segmented polyurethane and the excellent surface properties of a RF plasma polymer for the immobilization of a bioactive molecule is discussed. Specifically, glucose oxidase (GOx) has been covalently immobilized to poly(etherurethaneurea) (PEUU) films which were first modified with RF plasma polymerized N-vinyl-2-pyrrolidone (PPNVP). The results of the RIA and the immunochemical stain indicated that washes based on published procedures were inadequate in removing physically adsorbed GOx from the surfaces of PEUU and PPNVP/PEUU controls. The only wash which was effective consisted of continuous magnetic stirring in 900 ml of 2% sodium dodecyl sulfate, 2% Triton X-100, and 20 mM sodium phosphate (pH 7.0) for 20-24 hrs. each at 5°C.

AB - In this paper, the combination of the excellent bulk properties of a segmented polyurethane and the excellent surface properties of a RF plasma polymer for the immobilization of a bioactive molecule is discussed. Specifically, glucose oxidase (GOx) has been covalently immobilized to poly(etherurethaneurea) (PEUU) films which were first modified with RF plasma polymerized N-vinyl-2-pyrrolidone (PPNVP). The results of the RIA and the immunochemical stain indicated that washes based on published procedures were inadequate in removing physically adsorbed GOx from the surfaces of PEUU and PPNVP/PEUU controls. The only wash which was effective consisted of continuous magnetic stirring in 900 ml of 2% sodium dodecyl sulfate, 2% Triton X-100, and 20 mM sodium phosphate (pH 7.0) for 20-24 hrs. each at 5°C.

UR - http://www.scopus.com/inward/record.url?scp=0025789439&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025789439&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:0025789439

SP - 132

T2 - 17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium

Y2 - 1 May 1991 through 5 May 1991

ER -

Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea)

Abstract

Other

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this