TY - JOUR
T1 - Imaging mobile zinc in biology
AU - Tomat, Elisa
AU - Lippard, Stephen J.
N1 - Funding Information:
We acknowledge support from the National Institute of General Medical Sciences in the form of grant GM065519 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences or the National Institutes of Health.
PY - 2010/4
Y1 - 2010/4
N2 - Trafficking and regulation of mobile zinc pools influence cellular functions and pathological conditions in multiple organs, including brain, pancreas, and prostate. The quest for a dynamic description of zinc distribution and mobilization in live cells fuels the development of increasingly sophisticated probes. Detection systems that respond to zinc binding with changes of their fluorescence emission properties have provided sensitive tools for mobile zinc imaging, and fluorescence microscopy experiments have afforded depictions of zinc distribution within live cells and tissues. Both small-molecule and protein-based fluorescent probes can address complex imaging challenges, such as analyte quantification, site-specific sensor localization, and real-time detection.
AB - Trafficking and regulation of mobile zinc pools influence cellular functions and pathological conditions in multiple organs, including brain, pancreas, and prostate. The quest for a dynamic description of zinc distribution and mobilization in live cells fuels the development of increasingly sophisticated probes. Detection systems that respond to zinc binding with changes of their fluorescence emission properties have provided sensitive tools for mobile zinc imaging, and fluorescence microscopy experiments have afforded depictions of zinc distribution within live cells and tissues. Both small-molecule and protein-based fluorescent probes can address complex imaging challenges, such as analyte quantification, site-specific sensor localization, and real-time detection.
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U2 - 10.1016/j.cbpa.2009.12.010
DO - 10.1016/j.cbpa.2009.12.010
M3 - Review article
C2 - 20097117
AN - SCOPUS:77949903348
SN - 1367-5931
VL - 14
SP - 225
EP - 230
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
IS - 2
ER -