TY - JOUR
T1 - Identifying species of symbiont bacteria from the human gut that, alone, can induce intestinal Th17 cells in mice
AU - Tan, Tze Guan
AU - Sefik, Esen
AU - Geva-Zatorsky, Naama
AU - Kua, Lindsay
AU - Naskar, Debdut
AU - Teng, Fei
AU - Pasman, Lesley
AU - Ortiz-Lopez, Adriana
AU - Jupp, Ray
AU - Wu, Hsin Jung Joyce
AU - Kasper, Dennis L.
AU - Benoist, Christophe
AU - Mathis, Diane
N1 - Funding Information:
We thank Drs. A. Onderdonk and L. Bry for microbial strains; S. Edwards, A. T. Sherpa, J. Ramos, and K. Hattori for help with mice; A. Rhoads, L. Yang, and G. Gopalan for help with gene expression profiling and microbiota analysis; R. Bronson for help with scoring histopathology; and G. Buruzula and C. Araneo for help with cell sorting. This work was funded by a Sponsored Research Agreement from UCB (Union Chimique Belge) and NIH Grant R01AI107117 (to H.-J.J.W.). T.G.T. was supported by an Agency for Science, Technology, and Research Graduate Scholarship Fellowship. E.S. was supported by a fellowship from the Boehringer Ingelheim Fonds. N.G.-Z. was supported by Human Frontier Science Program Fellowship LT00079/2012, European Molecular Biology Organization Fellowship ALTF 251-2011, a Fulbright Award, a United Nations Educational, Scientific, and Cultural Organization L'Oreal National and International Women in Science Award, and the Weizmann Institute of Science National Postdoctoral Award Program for Advancing Women in Science. L.K. was supported by the National Science Foundation.
PY - 2016/12/13
Y1 - 2016/12/13
N2 - Th17 cells accrue in the intestine in response to particular microbes. In rodents, segmented filamentous bacteria (SFB) induce intestinal Th17 cells, but analogously functioning microbes in humans remain undefined. Here, we identified human symbiont bacterial species, in particular Bifidobacterium adolescentis, that could, alone, induce Th17 cells in the murine intestine. Similar to SFB, B. adolescentis was closely associated with the gut epithelium and engendered cognate Th17 cells without attendant inflammation. However, B. adolescentis elicited a transcriptional program clearly distinct from that of SFB, suggesting an alternative mechanism of promoting Th17 cell accumulation. Inoculation of mice with B. adolescentis exacerbated autoimmune arthritis in the K/BxN mouse model. Several off-the-shelf probiotic preparations that include Bifidobacterium strains also drove intestinal Th17 cell accumulation.
AB - Th17 cells accrue in the intestine in response to particular microbes. In rodents, segmented filamentous bacteria (SFB) induce intestinal Th17 cells, but analogously functioning microbes in humans remain undefined. Here, we identified human symbiont bacterial species, in particular Bifidobacterium adolescentis, that could, alone, induce Th17 cells in the murine intestine. Similar to SFB, B. adolescentis was closely associated with the gut epithelium and engendered cognate Th17 cells without attendant inflammation. However, B. adolescentis elicited a transcriptional program clearly distinct from that of SFB, suggesting an alternative mechanism of promoting Th17 cell accumulation. Inoculation of mice with B. adolescentis exacerbated autoimmune arthritis in the K/BxN mouse model. Several off-the-shelf probiotic preparations that include Bifidobacterium strains also drove intestinal Th17 cell accumulation.
KW - Intestine
KW - Microbiota
KW - Mucosal immunology
KW - Probiotic
KW - Th17 cells
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U2 - 10.1073/pnas.1617460113
DO - 10.1073/pnas.1617460113
M3 - Article
C2 - 27911839
AN - SCOPUS:85003426944
SN - 0027-8424
VL - 113
SP - E8141-E8150
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 50
ER -