TY - JOUR
T1 - Identification of the in vivo and in vitro origin of transcription in human rDNA
AU - Miesfeld, Roger
AU - Arnheim, Norman
N1 - Funding Information:
ACKNOWLEDGEMENTS We would like to thank Dr. J. Slightan for the human bacteriophage library, Dr. P. Hearing for the Adenovirus 5 Elb promoter containing clone, A. Lewis for providing the Hela cell culture, DTS . B. Sollner-Webb and R. Tijan for communicating unpublished results and D. Ryan for typing the manuscript. We also acknowledge the assistance of D. Davison with the computer analysis of the sequence homologies. This research was supported by a grant from the NIGMS.
PY - 1982/7/10
Y1 - 1982/7/10
N2 - A Hela cell S-100 extract primed with a purified human rDNA containing clone, has been shown to be capable of initiating specific α-amanitin-resistent RNA transcripts. By using a number of truncated templates, the site of RNA polymerase I initiation in vitro has been identified. The origin of transcription in vitro and in vivo was further defined by S1-mapping studies with total Hela cell RNA or RNA isolated from the in vitro transcription reaction. The initiation site was found to be the same. The nucleotide sequence of an 848 bp region around the initiation site, has also been determined. A perfect 15 bp homology has been found to exist between human and mouse rDNA very close to the origin of transcription, although little homology exists elsewhere. Sequences homolgous to the origin of transcription region were not found repeated within a 12 kb non-transcribed spacer segment upstream from it.
AB - A Hela cell S-100 extract primed with a purified human rDNA containing clone, has been shown to be capable of initiating specific α-amanitin-resistent RNA transcripts. By using a number of truncated templates, the site of RNA polymerase I initiation in vitro has been identified. The origin of transcription in vitro and in vivo was further defined by S1-mapping studies with total Hela cell RNA or RNA isolated from the in vitro transcription reaction. The initiation site was found to be the same. The nucleotide sequence of an 848 bp region around the initiation site, has also been determined. A perfect 15 bp homology has been found to exist between human and mouse rDNA very close to the origin of transcription, although little homology exists elsewhere. Sequences homolgous to the origin of transcription region were not found repeated within a 12 kb non-transcribed spacer segment upstream from it.
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U2 - 10.1093/nar/10.13.3933
DO - 10.1093/nar/10.13.3933
M3 - Article
C2 - 6287426
AN - SCOPUS:0020479245
SN - 0305-1048
VL - 10
SP - 3933
EP - 3939
JO - Nucleic acids research
JF - Nucleic acids research
IS - 13
ER -