Identification of prolactin receptors in hepatic nuclei

Arthur R. Buckley; David W. Montgomery; Mary J.C. Hendrix; Charles F. Zukoski; Charles W. Putnam

doi:10.1016/0003-9861(92)90563-C

Identification of prolactin receptors in hepatic nuclei

Arthur R. Buckley, David W. Montgomery, Mary J.C. Hendrix, Charles F. Zukoski, Charles W. Putnam

Surgery

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Prolactin is a trophic hormone which may act directly at the hepatocyte nucleus. In this study, specific prolactin binding sites were sought in purified rat liver nuclei. Saturable and specific, high affinity ¹²⁵I-prolactin binding sites were demonstrated to be on or within the nucleus. Prolactin binding was competitively inhibited by rat and ovine prolactins but not by rat growth hormone. Using immunogold electron microscopy, we detected prolactin receptors throughout the nucleus, in association with heterochromatin. Furthermore, endogenous immunoreactive prolactin was demonstrated to be within hepatic nuclei. We conclude that rat liver nuclei possess prolactin binding sites which likely participate in hormone-directed growth processes.

Original language	English (US)
Pages (from-to)	198-206
Number of pages	9
Journal	Archives of Biochemistry and Biophysics
Volume	296
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(92)90563-C
State	Published - Jul 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

Access to Document

10.1016/0003-9861(92)90563-C

Cite this

@article{a6e1d71455554ffca5942b38fcf09827,

title = "Identification of prolactin receptors in hepatic nuclei",

abstract = "Prolactin is a trophic hormone which may act directly at the hepatocyte nucleus. In this study, specific prolactin binding sites were sought in purified rat liver nuclei. Saturable and specific, high affinity 125I-prolactin binding sites were demonstrated to be on or within the nucleus. Prolactin binding was competitively inhibited by rat and ovine prolactins but not by rat growth hormone. Using immunogold electron microscopy, we detected prolactin receptors throughout the nucleus, in association with heterochromatin. Furthermore, endogenous immunoreactive prolactin was demonstrated to be within hepatic nuclei. We conclude that rat liver nuclei possess prolactin binding sites which likely participate in hormone-directed growth processes.",

author = "Buckley, {Arthur R.} and Montgomery, {David W.} and Hendrix, {Mary J.C.} and Zukoski, {Charles F.} and Putnam, {Charles W.}",

note = "Funding Information: We thank Dr. David W. Hein for his expertise and assistance with the determination of 2-acetylaminotluorene deacetylase. This work was supported by USPHS Grants DK40519 (D.W.M.), CA54984 (M.J.C.H.), and DK42279 (C.W.P.); by the Arizona Disease Control Research Commission (D.W.M., C.W.P.); and by Department of Veterans Affairs Merit Review Grants (D.W.M., C.W.P., C.F.Z.), the PMA Foundation, and the NSF ASEND Program (A.R.B.).",

year = "1992",

month = jul,

doi = "10.1016/0003-9861(92)90563-C",

language = "English (US)",

volume = "296",

pages = "198--206",

journal = "Archives of Biochemistry and Biophysics",

issn = "0003-9861",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Identification of prolactin receptors in hepatic nuclei

AU - Buckley, Arthur R.

AU - Montgomery, David W.

AU - Hendrix, Mary J.C.

AU - Zukoski, Charles F.

AU - Putnam, Charles W.

N1 - Funding Information: We thank Dr. David W. Hein for his expertise and assistance with the determination of 2-acetylaminotluorene deacetylase. This work was supported by USPHS Grants DK40519 (D.W.M.), CA54984 (M.J.C.H.), and DK42279 (C.W.P.); by the Arizona Disease Control Research Commission (D.W.M., C.W.P.); and by Department of Veterans Affairs Merit Review Grants (D.W.M., C.W.P., C.F.Z.), the PMA Foundation, and the NSF ASEND Program (A.R.B.).

PY - 1992/7

Y1 - 1992/7

N2 - Prolactin is a trophic hormone which may act directly at the hepatocyte nucleus. In this study, specific prolactin binding sites were sought in purified rat liver nuclei. Saturable and specific, high affinity 125I-prolactin binding sites were demonstrated to be on or within the nucleus. Prolactin binding was competitively inhibited by rat and ovine prolactins but not by rat growth hormone. Using immunogold electron microscopy, we detected prolactin receptors throughout the nucleus, in association with heterochromatin. Furthermore, endogenous immunoreactive prolactin was demonstrated to be within hepatic nuclei. We conclude that rat liver nuclei possess prolactin binding sites which likely participate in hormone-directed growth processes.

AB - Prolactin is a trophic hormone which may act directly at the hepatocyte nucleus. In this study, specific prolactin binding sites were sought in purified rat liver nuclei. Saturable and specific, high affinity 125I-prolactin binding sites were demonstrated to be on or within the nucleus. Prolactin binding was competitively inhibited by rat and ovine prolactins but not by rat growth hormone. Using immunogold electron microscopy, we detected prolactin receptors throughout the nucleus, in association with heterochromatin. Furthermore, endogenous immunoreactive prolactin was demonstrated to be within hepatic nuclei. We conclude that rat liver nuclei possess prolactin binding sites which likely participate in hormone-directed growth processes.

UR - http://www.scopus.com/inward/record.url?scp=0026631732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026631732&partnerID=8YFLogxK

U2 - 10.1016/0003-9861(92)90563-C

DO - 10.1016/0003-9861(92)90563-C

M3 - Article

C2 - 1605631

AN - SCOPUS:0026631732

SN - 0003-9861

VL - 296

SP - 198

EP - 206

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

IS - 1

ER -

Identification of prolactin receptors in hepatic nuclei

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this