Identification of methylated transcripts using the TRIBE approach

Lina Worpenberg, Tobias Jakobi, Christoph Dieterich, Jean Yves Roignant

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations


m6A is the most abundant internal modification on mRNA. Recent improvements of high-throughput sequencing techniques enables its detection at the transcriptome level, even at the nucleotide resolution. However most current techniques require large amounts of starting material to detect the modification. Here, we describe a complementary technique of standard meRIP-seq/miCLIP-seq approaches to identify methylated RNA using a low amount of material. We believe this approach can be applied in vivo to identify methylated targets in specific tissues or subpopulations of cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages18
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • dAdar
  • Editing
  • mA
  • mRNA modification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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