Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA

Carika Weldon; Isabelle Behm-Ansmant; Laurence H. Hurley; Glenn A. Burley; Christiane Branlant; Ian C. Eperon; Cyril Dominguez

doi:10.1038/nchembio.2228

Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA

Carika Weldon
, Isabelle Behm-Ansmant
, Laurence H. Hurley
, Glenn A. Burley
, Christiane Branlant
, Ian C. Eperon
, Cyril Dominguez

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.

Original language	English (US)
Pages (from-to)	18-20
Number of pages	3
Journal	Nature chemical biology
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/nchembio.2228
State	Published - Jan 1 2017

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1038/nchembio.2228

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abstract = "RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.",

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AU - Burley, Glenn A.

AU - Branlant, Christiane

AU - Eperon, Ian C.

AU - Dominguez, Cyril

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AB - RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.

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Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA

Abstract

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