TY - JOUR
T1 - Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA
AU - Weldon, Carika
AU - Behm-Ansmant, Isabelle
AU - Hurley, Laurence H.
AU - Burley, Glenn A.
AU - Branlant, Christiane
AU - Eperon, Ian C.
AU - Dominguez, Cyril
N1 - Publisher Copyright:
© 2016 Nature America, Inc. All rights reserved.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.
AB - RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.
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U2 - 10.1038/nchembio.2228
DO - 10.1038/nchembio.2228
M3 - Article
C2 - 27820800
AN - SCOPUS:84994684079
SN - 1552-4450
VL - 13
SP - 18
EP - 20
JO - Nature chemical biology
JF - Nature chemical biology
IS - 1
ER -