Identification of B2 bradykinin binding sites in the guinea pig nasal turbinate

Yutaka Fujiwara; John W. Bloom; Charles R. Mantione; Henry I. Yamamura

doi:10.1016/0196-9781(89)90163-0

Identification of B₂ bradykinin binding sites in the guinea pig nasal turbinate

Yutaka Fujiwara, John W. Bloom, Charles R. Mantione, Henry I. Yamamura

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Specific high affinity BK binding sites in the nasal turbinate of the guinea pig have been demonstrated. Specific [³H]BK binding (10-330 pM) was saturable, and nonlinear least squares analysis indicated the presence of a high affinity binding site with a K_d value of 60 (50-78) pM and a B_max value of 13.1±2.0 fmol/mg protein. In inhibition experiments, D-Phe⁷-BK (a B₂ antagonist) inhibited [³H]BK binding with a K_i value of 23 nM, while des-Arg⁹[Leu⁸]-BK (a B₁ antagonist) had no effect up to a concentration of 10 μM. These studies indicate the presence of B₂ BK receptors in the guinea pig nasal turbinate.

Original language	English (US)
Pages (from-to)	701-703
Number of pages	3
Journal	Peptides
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/0196-9781(89)90163-0
State	Published - 1989

Keywords

B bradykinin receptor
Nasal turbinate
[H]Bradykinin binding

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/0196-9781(89)90163-0

Cite this

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title = "Identification of B2 bradykinin binding sites in the guinea pig nasal turbinate",

abstract = "Specific high affinity BK binding sites in the nasal turbinate of the guinea pig have been demonstrated. Specific [3H]BK binding (10-330 pM) was saturable, and nonlinear least squares analysis indicated the presence of a high affinity binding site with a Kd value of 60 (50-78) pM and a Bmax value of 13.1±2.0 fmol/mg protein. In inhibition experiments, D-Phe7-BK (a B2 antagonist) inhibited [3H]BK binding with a Ki value of 23 nM, while des-Arg9[Leu8]-BK (a B1 antagonist) had no effect up to a concentration of 10 μM. These studies indicate the presence of B2 BK receptors in the guinea pig nasal turbinate.",

keywords = "B bradykinin receptor, Nasal turbinate, [H]Bradykinin binding",

author = "Yutaka Fujiwara and Bloom, {John W.} and Mantione, {Charles R.} and Yamamura, {Henry I.}",

note = "Funding Information: Supported in part by a U.S.P.H.S. Research Commission.",

year = "1989",

doi = "10.1016/0196-9781(89)90163-0",

language = "English (US)",

volume = "10",

pages = "701--703",

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T1 - Identification of B2 bradykinin binding sites in the guinea pig nasal turbinate

AU - Fujiwara, Yutaka

AU - Bloom, John W.

AU - Mantione, Charles R.

AU - Yamamura, Henry I.

N1 - Funding Information: Supported in part by a U.S.P.H.S. Research Commission.

PY - 1989

Y1 - 1989

N2 - Specific high affinity BK binding sites in the nasal turbinate of the guinea pig have been demonstrated. Specific [3H]BK binding (10-330 pM) was saturable, and nonlinear least squares analysis indicated the presence of a high affinity binding site with a Kd value of 60 (50-78) pM and a Bmax value of 13.1±2.0 fmol/mg protein. In inhibition experiments, D-Phe7-BK (a B2 antagonist) inhibited [3H]BK binding with a Ki value of 23 nM, while des-Arg9[Leu8]-BK (a B1 antagonist) had no effect up to a concentration of 10 μM. These studies indicate the presence of B2 BK receptors in the guinea pig nasal turbinate.

AB - Specific high affinity BK binding sites in the nasal turbinate of the guinea pig have been demonstrated. Specific [3H]BK binding (10-330 pM) was saturable, and nonlinear least squares analysis indicated the presence of a high affinity binding site with a Kd value of 60 (50-78) pM and a Bmax value of 13.1±2.0 fmol/mg protein. In inhibition experiments, D-Phe7-BK (a B2 antagonist) inhibited [3H]BK binding with a Ki value of 23 nM, while des-Arg9[Leu8]-BK (a B1 antagonist) had no effect up to a concentration of 10 μM. These studies indicate the presence of B2 BK receptors in the guinea pig nasal turbinate.

KW - B bradykinin receptor

KW - Nasal turbinate

KW - [H]Bradykinin binding

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