Identification of adenylyl cyclase isoenzymes in CHO and B82 cells

Eva V. Varga; Dagmar Stropova; Marc Rubenzik; Man Wang; Robert S. Landsman; William R. Roeske; Henry I. Yamamura

doi:10.1016/S0014-2999(98)00258-1

Identification of adenylyl cyclase isoenzymes in CHO and B82 cells

Eva V. Varga, Dagmar Stropova, Marc Rubenzik, Man Wang, Robert S. Landsman, William R. Roeske, Henry I. Yamamura

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43 Scopus citations

Abstract

The identification of adenylyl cyclase isoenzymes in mammalian host cells is important for the interpretation of data obtained from cell lines heterologously expressing G-protein coupled receptors. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify adenylyl cyclase cDNAs from Chinese hamster ovary (CHO) and mouse fibroblast (B82) cells. The isolated fragments were identified by restriction analyses and by sequencing. We found mRNAs for adenylyl cyclases VI and VII in CHO and adenylyl cyclases IX and VII in B82 cells.

Original language	English (US)
Pages (from-to)	R1-R2
Journal	European Journal of Pharmacology
Volume	348
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-2999(98)00258-1
State	Published - May 8 1998

Keywords

Adenylyl cyclase isoenzyme
Chinese hamster ovary (CHO) cell
Mouse fibroblast (B82) cell

ASJC Scopus subject areas

Pharmacology

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10.1016/S0014-2999(98)00258-1

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title = "Identification of adenylyl cyclase isoenzymes in CHO and B82 cells",

abstract = "The identification of adenylyl cyclase isoenzymes in mammalian host cells is important for the interpretation of data obtained from cell lines heterologously expressing G-protein coupled receptors. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify adenylyl cyclase cDNAs from Chinese hamster ovary (CHO) and mouse fibroblast (B82) cells. The isolated fragments were identified by restriction analyses and by sequencing. We found mRNAs for adenylyl cyclases VI and VII in CHO and adenylyl cyclases IX and VII in B82 cells.",

keywords = "Adenylyl cyclase isoenzyme, Chinese hamster ovary (CHO) cell, Mouse fibroblast (B82) cell",

author = "Varga, {Eva V.} and Dagmar Stropova and Marc Rubenzik and Man Wang and Landsman, {Robert S.} and Roeske, {William R.} and Yamamura, {Henry I.}",

note = "Funding Information: This work was supported in parts by grants from the Arizona Disease Control Research Commission, NIDA and NHLBI.",

year = "1998",

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doi = "10.1016/S0014-2999(98)00258-1",

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TY - JOUR

T1 - Identification of adenylyl cyclase isoenzymes in CHO and B82 cells

AU - Varga, Eva V.

AU - Stropova, Dagmar

AU - Rubenzik, Marc

AU - Wang, Man

AU - Landsman, Robert S.

AU - Roeske, William R.

AU - Yamamura, Henry I.

N1 - Funding Information: This work was supported in parts by grants from the Arizona Disease Control Research Commission, NIDA and NHLBI.

PY - 1998/5/8

Y1 - 1998/5/8

N2 - The identification of adenylyl cyclase isoenzymes in mammalian host cells is important for the interpretation of data obtained from cell lines heterologously expressing G-protein coupled receptors. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify adenylyl cyclase cDNAs from Chinese hamster ovary (CHO) and mouse fibroblast (B82) cells. The isolated fragments were identified by restriction analyses and by sequencing. We found mRNAs for adenylyl cyclases VI and VII in CHO and adenylyl cyclases IX and VII in B82 cells.

AB - The identification of adenylyl cyclase isoenzymes in mammalian host cells is important for the interpretation of data obtained from cell lines heterologously expressing G-protein coupled receptors. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify adenylyl cyclase cDNAs from Chinese hamster ovary (CHO) and mouse fibroblast (B82) cells. The isolated fragments were identified by restriction analyses and by sequencing. We found mRNAs for adenylyl cyclases VI and VII in CHO and adenylyl cyclases IX and VII in B82 cells.

KW - Adenylyl cyclase isoenzyme

KW - Chinese hamster ovary (CHO) cell

KW - Mouse fibroblast (B82) cell

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JO - European Journal of Pharmacology

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Identification of adenylyl cyclase isoenzymes in CHO and B82 cells

Abstract

Keywords

ASJC Scopus subject areas

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