Identification and distribution of endoplasmic reticulum iPLA 2

Gilbert R. Kinsey; Brian S. Cummings; Caroline S. Beckett; Geraldine Saavedra; Wenliang Zhang; Jane McHowat; Rick G. Schnellmann

doi:10.1016/j.bbrc.2004.12.016

Identification and distribution of endoplasmic reticulum iPLA ₂

Gilbert R. Kinsey, Brian S. Cummings, Caroline S. Beckett, Geraldine Saavedra, Wenliang Zhang, Jane McHowat, Rick G. Schnellmann

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Our laboratory demonstrated that endoplasmic reticulum iPLA ₂ (ER-iPLA ₂) activity protects renal cells from oxidant-induced cell death and lipid peroxidation. The goals of this study were to determine the PLA ₂ isoform(s) responsible for ER-iPLA ₂ activity in different species and tissues. ER-iPLA ₂ activity was observed in microsomes from rabbit and rat kidney, heart, and brain as well as in human kidney (Caki-1 and HEK293) and glioblastoma (A172) cell lines. Reverse transcriptase-polymerase chain reaction results demonstrated the presence of iPLA ₂γ (group VIB PLA ₂) message in all tissues tested. Immunoblot analysis and PLA ₂ inhibitor studies with methyl arachidonyl fluorophosphonate and enantiomers of bromoenol lactone demonstrated that the ER-iPLA ₂ in rabbit kidney and heart and rat kidney is iPLA ₂γ. These results demonstrate the expression of ER-iPLA ₂γ (group VIB) across species and tissues, and suggest that iPLA ₂γ may play critical roles in oxidant-induced cell injury.

Original language	English (US)
Pages (from-to)	287-293
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	327
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2004.12.016
State	Published - Feb 4 2005
Externally published	Yes

Keywords

Endoplasmic reticulum
Group IVC PLA
Group VIB PLA
cPLA γ
iPLA γ

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2004.12.016

Cite this

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title = "Identification and distribution of endoplasmic reticulum iPLA 2 ",

abstract = "Our laboratory demonstrated that endoplasmic reticulum iPLA 2 (ER-iPLA 2) activity protects renal cells from oxidant-induced cell death and lipid peroxidation. The goals of this study were to determine the PLA 2 isoform(s) responsible for ER-iPLA 2 activity in different species and tissues. ER-iPLA 2 activity was observed in microsomes from rabbit and rat kidney, heart, and brain as well as in human kidney (Caki-1 and HEK293) and glioblastoma (A172) cell lines. Reverse transcriptase-polymerase chain reaction results demonstrated the presence of iPLA 2γ (group VIB PLA 2) message in all tissues tested. Immunoblot analysis and PLA 2 inhibitor studies with methyl arachidonyl fluorophosphonate and enantiomers of bromoenol lactone demonstrated that the ER-iPLA 2 in rabbit kidney and heart and rat kidney is iPLA 2γ. These results demonstrate the expression of ER-iPLA 2γ (group VIB) across species and tissues, and suggest that iPLA 2γ may play critical roles in oxidant-induced cell injury.",

keywords = "Endoplasmic reticulum, Group IVC PLA, Group VIB PLA, cPLA γ, iPLA γ",

author = "Kinsey, \{Gilbert R.\} and Cummings, \{Brian S.\} and Beckett, \{Caroline S.\} and Geraldine Saavedra and Wenliang Zhang and Jane McHowat and Schnellmann, \{Rick G.\}",

note = "Funding Information: This work was supported by a National Research Service Award DK-10079 (to B.S.C.) and a National Institutes of Health Grant DK-62028 (to R.G.S. and J.M.).",

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AU - Kinsey, Gilbert R.

AU - Cummings, Brian S.

AU - Beckett, Caroline S.

AU - Saavedra, Geraldine

AU - Zhang, Wenliang

AU - McHowat, Jane

AU - Schnellmann, Rick G.

N1 - Funding Information: This work was supported by a National Research Service Award DK-10079 (to B.S.C.) and a National Institutes of Health Grant DK-62028 (to R.G.S. and J.M.).

PY - 2005/2/4

Y1 - 2005/2/4

N2 - Our laboratory demonstrated that endoplasmic reticulum iPLA 2 (ER-iPLA 2) activity protects renal cells from oxidant-induced cell death and lipid peroxidation. The goals of this study were to determine the PLA 2 isoform(s) responsible for ER-iPLA 2 activity in different species and tissues. ER-iPLA 2 activity was observed in microsomes from rabbit and rat kidney, heart, and brain as well as in human kidney (Caki-1 and HEK293) and glioblastoma (A172) cell lines. Reverse transcriptase-polymerase chain reaction results demonstrated the presence of iPLA 2γ (group VIB PLA 2) message in all tissues tested. Immunoblot analysis and PLA 2 inhibitor studies with methyl arachidonyl fluorophosphonate and enantiomers of bromoenol lactone demonstrated that the ER-iPLA 2 in rabbit kidney and heart and rat kidney is iPLA 2γ. These results demonstrate the expression of ER-iPLA 2γ (group VIB) across species and tissues, and suggest that iPLA 2γ may play critical roles in oxidant-induced cell injury.

AB - Our laboratory demonstrated that endoplasmic reticulum iPLA 2 (ER-iPLA 2) activity protects renal cells from oxidant-induced cell death and lipid peroxidation. The goals of this study were to determine the PLA 2 isoform(s) responsible for ER-iPLA 2 activity in different species and tissues. ER-iPLA 2 activity was observed in microsomes from rabbit and rat kidney, heart, and brain as well as in human kidney (Caki-1 and HEK293) and glioblastoma (A172) cell lines. Reverse transcriptase-polymerase chain reaction results demonstrated the presence of iPLA 2γ (group VIB PLA 2) message in all tissues tested. Immunoblot analysis and PLA 2 inhibitor studies with methyl arachidonyl fluorophosphonate and enantiomers of bromoenol lactone demonstrated that the ER-iPLA 2 in rabbit kidney and heart and rat kidney is iPLA 2γ. These results demonstrate the expression of ER-iPLA 2γ (group VIB) across species and tissues, and suggest that iPLA 2γ may play critical roles in oxidant-induced cell injury.

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KW - Group VIB PLA

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KW - iPLA γ

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