Identification and distribution of endoplasmic reticulum iPLA 2

Gilbert R. Kinsey, Brian S. Cummings, Caroline S. Beckett, Geraldine Saavedra, Wenliang Zhang, Jane McHowat, Rick G. Schnellmann

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Our laboratory demonstrated that endoplasmic reticulum iPLA 2 (ER-iPLA 2) activity protects renal cells from oxidant-induced cell death and lipid peroxidation. The goals of this study were to determine the PLA 2 isoform(s) responsible for ER-iPLA 2 activity in different species and tissues. ER-iPLA 2 activity was observed in microsomes from rabbit and rat kidney, heart, and brain as well as in human kidney (Caki-1 and HEK293) and glioblastoma (A172) cell lines. Reverse transcriptase-polymerase chain reaction results demonstrated the presence of iPLA 2γ (group VIB PLA 2) message in all tissues tested. Immunoblot analysis and PLA 2 inhibitor studies with methyl arachidonyl fluorophosphonate and enantiomers of bromoenol lactone demonstrated that the ER-iPLA 2 in rabbit kidney and heart and rat kidney is iPLA 2γ. These results demonstrate the expression of ER-iPLA 2γ (group VIB) across species and tissues, and suggest that iPLA 2γ may play critical roles in oxidant-induced cell injury.

Original languageEnglish (US)
Pages (from-to)287-293
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume327
Issue number1
DOIs
StatePublished - Feb 4 2005
Externally publishedYes

Keywords

  • cPLA γ
  • Endoplasmic reticulum
  • Group IVC PLA
  • Group VIB PLA
  • iPLA γ

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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