I. High performance liquid chromatography of pharmacologically active amines and peptides in biological materials

Thomas P. Davis; Hans Schoemaker; Andrew Chen; Henry I. Yamamura

doi:10.1016/0024-3205(82)90515-X

I. High performance liquid chromatography of pharmacologically active amines and peptides in biological materials

Thomas P. Davis, Hans Schoemaker, Andrew Chen, Henry I. Yamamura

Research output: Contribution to journal › Short survey › peer-review

21 Scopus citations

Abstract

Precise and quantitative reversed-phase high performance liquid chromatographic (HPLC) procedures are described which can be used in biogenic amine and neuropeptide research. The amine procedure was applied to various pharmacological matrices including plasma, heart tissue and brain. The use of peptide HPLC as an analytical tool for various neuropeptides is illustrated by studies on des-tyrosine-γ-endorphin (DTγE) metabolism in the brain and the stability of an ACTH (ORG-2766) analogue during a chronic infusion in rats. The power of HPLC as a research tool in peptide pharmacology is described, discussed and demonstrated as an aid in the understanding of the pharmacological effects of exogenous peptides and the function of the brain.

Original language	English (US)
Pages (from-to)	971-987
Number of pages	17
Journal	Life Sciences
Volume	30
Issue number	12
DOIs	https://doi.org/10.1016/0024-3205(82)90515-X
State	Published - Mar 22 1982

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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@article{168c71c821eb463bb9a5443860a57302,

title = "I. High performance liquid chromatography of pharmacologically active amines and peptides in biological materials",

abstract = "Precise and quantitative reversed-phase high performance liquid chromatographic (HPLC) procedures are described which can be used in biogenic amine and neuropeptide research. The amine procedure was applied to various pharmacological matrices including plasma, heart tissue and brain. The use of peptide HPLC as an analytical tool for various neuropeptides is illustrated by studies on des-tyrosine-γ-endorphin (DTγE) metabolism in the brain and the stability of an ACTH (ORG-2766) analogue during a chronic infusion in rats. The power of HPLC as a research tool in peptide pharmacology is described, discussed and demonstrated as an aid in the understanding of the pharmacological effects of exogenous peptides and the function of the brain.",

author = "Davis, {Thomas P.} and Hans Schoemaker and Andrew Chen and Yamamura, {Henry I.}",

note = "Funding Information: This research was supported by USPHS Grants MH-27257 and MH-30626 to Henry I. Yamamura and a BRSG Grant to Thomas P. Davis. Henry I. Yamamura is a recipient of a USPHS RSDA (M}{-00095) from the NIMH. The authors wish to acknowledge the excellent art work of Mrs. Wendy McFadden and skillful typing of Ms. Pat Lambotte and Ms. Jackie McConachie.",

year = "1982",

month = mar,

day = "22",

doi = "10.1016/0024-3205(82)90515-X",

language = "English (US)",

volume = "30",

pages = "971--987",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "12",

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TY - JOUR

T1 - I. High performance liquid chromatography of pharmacologically active amines and peptides in biological materials

AU - Davis, Thomas P.

AU - Schoemaker, Hans

AU - Chen, Andrew

AU - Yamamura, Henry I.

N1 - Funding Information: This research was supported by USPHS Grants MH-27257 and MH-30626 to Henry I. Yamamura and a BRSG Grant to Thomas P. Davis. Henry I. Yamamura is a recipient of a USPHS RSDA (M}{-00095) from the NIMH. The authors wish to acknowledge the excellent art work of Mrs. Wendy McFadden and skillful typing of Ms. Pat Lambotte and Ms. Jackie McConachie.

PY - 1982/3/22

Y1 - 1982/3/22

N2 - Precise and quantitative reversed-phase high performance liquid chromatographic (HPLC) procedures are described which can be used in biogenic amine and neuropeptide research. The amine procedure was applied to various pharmacological matrices including plasma, heart tissue and brain. The use of peptide HPLC as an analytical tool for various neuropeptides is illustrated by studies on des-tyrosine-γ-endorphin (DTγE) metabolism in the brain and the stability of an ACTH (ORG-2766) analogue during a chronic infusion in rats. The power of HPLC as a research tool in peptide pharmacology is described, discussed and demonstrated as an aid in the understanding of the pharmacological effects of exogenous peptides and the function of the brain.

AB - Precise and quantitative reversed-phase high performance liquid chromatographic (HPLC) procedures are described which can be used in biogenic amine and neuropeptide research. The amine procedure was applied to various pharmacological matrices including plasma, heart tissue and brain. The use of peptide HPLC as an analytical tool for various neuropeptides is illustrated by studies on des-tyrosine-γ-endorphin (DTγE) metabolism in the brain and the stability of an ACTH (ORG-2766) analogue during a chronic infusion in rats. The power of HPLC as a research tool in peptide pharmacology is described, discussed and demonstrated as an aid in the understanding of the pharmacological effects of exogenous peptides and the function of the brain.

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U2 - 10.1016/0024-3205(82)90515-X

DO - 10.1016/0024-3205(82)90515-X

M3 - Short survey

C2 - 6280002

AN - SCOPUS:0020071124

SN - 0024-3205

VL - 30

SP - 971

EP - 987

JO - Life Sciences

JF - Life Sciences

IS - 12

ER -

I. High performance liquid chromatography of pharmacologically active amines and peptides in biological materials

Abstract

ASJC Scopus subject areas

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