Abstract
A new microscope is discussed, where the scanning illumination has a numerical aperture of 2.8 with λ = 1.56 μm femtosecond fiber laser. Samples are placed or grown on a silicon substrate. Multi-photon emission is imaged in transmission on a cooled CCD. Two-photon and three-photon effects are observed from the silicon/water interface and gold nanoparticles. Images of cells, reference spheres and gold nanoparticles illustrate imaging properties of the microscope. Spectral characteristics of individual particles are achieved with a blazed transmission grating. Emission properties of differently sized gold nanoparticles are studied in detail, which indicate that their emission is a two-photon effect due continuum generation. Interestingly, spectral shape and emission power are similar for 20nm, 40nm and 60nm diameter gold nanoparticles for the cases studied.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1786-1794 |
| Number of pages | 9 |
| Journal | Biomedical Optics Express |
| Volume | 4 |
| Issue number | 10 |
| DOIs | |
| State | Published - 2013 |
ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics
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