TY - JOUR
T1 - Hyper-numerical aperture (NA = 2.8) microscope using λ = 1.56 μm femtosecond source for multi-photon imaging
AU - Kim, Youngsik
AU - Lu, Phat
AU - Milster, Tom D.
AU - Kieu, Khanh
PY - 2013
Y1 - 2013
N2 - A new microscope is discussed, where the scanning illumination has a numerical aperture of 2.8 with λ = 1.56 μm femtosecond fiber laser. Samples are placed or grown on a silicon substrate. Multi-photon emission is imaged in transmission on a cooled CCD. Two-photon and three-photon effects are observed from the silicon/water interface and gold nanoparticles. Images of cells, reference spheres and gold nanoparticles illustrate imaging properties of the microscope. Spectral characteristics of individual particles are achieved with a blazed transmission grating. Emission properties of differently sized gold nanoparticles are studied in detail, which indicate that their emission is a two-photon effect due continuum generation. Interestingly, spectral shape and emission power are similar for 20nm, 40nm and 60nm diameter gold nanoparticles for the cases studied.
AB - A new microscope is discussed, where the scanning illumination has a numerical aperture of 2.8 with λ = 1.56 μm femtosecond fiber laser. Samples are placed or grown on a silicon substrate. Multi-photon emission is imaged in transmission on a cooled CCD. Two-photon and three-photon effects are observed from the silicon/water interface and gold nanoparticles. Images of cells, reference spheres and gold nanoparticles illustrate imaging properties of the microscope. Spectral characteristics of individual particles are achieved with a blazed transmission grating. Emission properties of differently sized gold nanoparticles are studied in detail, which indicate that their emission is a two-photon effect due continuum generation. Interestingly, spectral shape and emission power are similar for 20nm, 40nm and 60nm diameter gold nanoparticles for the cases studied.
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U2 - 10.1364/BOE.4.001786
DO - 10.1364/BOE.4.001786
M3 - Article
C2 - 24156043
AN - SCOPUS:84884961626
SN - 2156-7085
VL - 4
SP - 1786
EP - 1794
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 10
ER -