Humoral antibody responses to specific antigens of coccidioides immitis

David L. Calhoun; Ellie O. Osir; Kris O. Dugger; John N. Galgiani; John H. Law; David L. Calhoun; Ellie O. Osir; Kris O. Dugger; John N. Galgiani; John H. Law

doi:10.1093/infdis/154.2.265

Humoral antibody responses to specific antigens of coccidioides immitis

David L. Calhoun, Ellie O. Osir, Kris O. Dugger, John N. Galgiani, John H. Law, David L. Calhoun, Ellie O. Osir, Kris O. Dugger, John N. Galgiani, John H. Law

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

To identify antigens important to the diagnosis or pathogenesis of coccidioidomycosis, we analyzed three coccidioidal extracts by immunoblotting techniques with sera from patients infected with Coccidioides immitis and from immunized rabbits. When denatured fungal extracts were used, sera from at least 9 of 10 patients reacted with antigens of 100, 60, and 45 kilodaltons (kDa). By agar diffusion, the 100-kDa antigen appeared to be identical to the conventional tube precipitin antigen. Five of the six sera from patients with pulmonary syndromes reacted with another antigen of 70 kDa, but sera from patients with disseminated disease did not react with this antigen. Both the 100-kDa and the 70-kDa antigens were present in the 70-130-kDa region of the nondenatured fungal extracts. Our results suggest that the 100-kDa antigen may be useful in future diagnostic tests and that the 70-kDa antigen may identify patients whose infection is confined to the chest.

Original language	English (US)
Pages (from-to)	265-272
Number of pages	8
Journal	Journal of Infectious Diseases
Volume	154
Issue number	2
DOIs	https://doi.org/10.1093/infdis/154.2.265
State	Published - Aug 1986

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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@article{118640b830204a6a87571847a95bc454,

title = "Humoral antibody responses to specific antigens of coccidioides immitis",

abstract = "To identify antigens important to the diagnosis or pathogenesis of coccidioidomycosis, we analyzed three coccidioidal extracts by immunoblotting techniques with sera from patients infected with Coccidioides immitis and from immunized rabbits. When denatured fungal extracts were used, sera from at least 9 of 10 patients reacted with antigens of 100, 60, and 45 kilodaltons (kDa). By agar diffusion, the 100-kDa antigen appeared to be identical to the conventional tube precipitin antigen. Five of the six sera from patients with pulmonary syndromes reacted with another antigen of 70 kDa, but sera from patients with disseminated disease did not react with this antigen. Both the 100-kDa and the 70-kDa antigens were present in the 70-130-kDa region of the nondenatured fungal extracts. Our results suggest that the 100-kDa antigen may be useful in future diagnostic tests and that the 70-kDa antigen may identify patients whose infection is confined to the chest.",

author = "Calhoun, {David L.} and Osir, {Ellie O.} and Dugger, {Kris O.} and Galgiani, {John N.} and Law, {John H.} and Calhoun, {David L.} and Osir, {Ellie O.} and Dugger, {Kris O.} and Galgiani, {John N.} and Law, {John H.}",

note = "Funding Information: Received for publication 10 February 1986. This work was presented in part at the 25th Interscience Conference on Antimicrobial Agents and Chemotherapy, held in Minneapolis, Minnesota in October 1985. Informed consent was obtained from all participants in this study, and the study was conducted in accordance with the University of Arizona Human Subjects Research Committee guidelines for human experimentation. This work was supported in part by the Veterans Administration and the Arizona Kidney Foundation. We thank Rosemary Hayden for assistance in preparing the fungal antigens and Marion Wieden for performing the conventional immunodiffusion studies. Please address requests for reprints to Dr. John N. Galgiani, Medical Service (HI), Veterans Administration Medical Center, Tuscon, Arizona 85723.",

year = "1986",

month = aug,

doi = "10.1093/infdis/154.2.265",

language = "English (US)",

volume = "154",

pages = "265--272",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

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T1 - Humoral antibody responses to specific antigens of coccidioides immitis

AU - Calhoun, David L.

AU - Osir, Ellie O.

AU - Dugger, Kris O.

AU - Galgiani, John N.

AU - Law, John H.

AU - Calhoun, David L.

AU - Osir, Ellie O.

AU - Dugger, Kris O.

AU - Galgiani, John N.

AU - Law, John H.

N1 - Funding Information: Received for publication 10 February 1986. This work was presented in part at the 25th Interscience Conference on Antimicrobial Agents and Chemotherapy, held in Minneapolis, Minnesota in October 1985. Informed consent was obtained from all participants in this study, and the study was conducted in accordance with the University of Arizona Human Subjects Research Committee guidelines for human experimentation. This work was supported in part by the Veterans Administration and the Arizona Kidney Foundation. We thank Rosemary Hayden for assistance in preparing the fungal antigens and Marion Wieden for performing the conventional immunodiffusion studies. Please address requests for reprints to Dr. John N. Galgiani, Medical Service (HI), Veterans Administration Medical Center, Tuscon, Arizona 85723.

PY - 1986/8

Y1 - 1986/8

N2 - To identify antigens important to the diagnosis or pathogenesis of coccidioidomycosis, we analyzed three coccidioidal extracts by immunoblotting techniques with sera from patients infected with Coccidioides immitis and from immunized rabbits. When denatured fungal extracts were used, sera from at least 9 of 10 patients reacted with antigens of 100, 60, and 45 kilodaltons (kDa). By agar diffusion, the 100-kDa antigen appeared to be identical to the conventional tube precipitin antigen. Five of the six sera from patients with pulmonary syndromes reacted with another antigen of 70 kDa, but sera from patients with disseminated disease did not react with this antigen. Both the 100-kDa and the 70-kDa antigens were present in the 70-130-kDa region of the nondenatured fungal extracts. Our results suggest that the 100-kDa antigen may be useful in future diagnostic tests and that the 70-kDa antigen may identify patients whose infection is confined to the chest.

AB - To identify antigens important to the diagnosis or pathogenesis of coccidioidomycosis, we analyzed three coccidioidal extracts by immunoblotting techniques with sera from patients infected with Coccidioides immitis and from immunized rabbits. When denatured fungal extracts were used, sera from at least 9 of 10 patients reacted with antigens of 100, 60, and 45 kilodaltons (kDa). By agar diffusion, the 100-kDa antigen appeared to be identical to the conventional tube precipitin antigen. Five of the six sera from patients with pulmonary syndromes reacted with another antigen of 70 kDa, but sera from patients with disseminated disease did not react with this antigen. Both the 100-kDa and the 70-kDa antigens were present in the 70-130-kDa region of the nondenatured fungal extracts. Our results suggest that the 100-kDa antigen may be useful in future diagnostic tests and that the 70-kDa antigen may identify patients whose infection is confined to the chest.

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Humoral antibody responses to specific antigens of coccidioides immitis

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