Human laminin-5 and laminin-10 mediated gene expression of prostate carcinoma cells

Robert Calaluce, Shaleen K. Beck, Elisabeth L. Bair, Ritu Pandey, Kevin A. Greer, Adam M. Hoying, James B. Hoying, David W. Mount, Raymond B Nagle

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In prostate cancer progression, the basal lamina switches from predominantly laminin-5 to laminin-10. DU-145 prostate cancer cells were treated with either soluble laminin-5 (20 ng/ml) or laminin-10 (1 μg/ml) for 6, 24, and 48 hr. Total RNA was harvested for a 7,500 human cDNA microarray. Hybridizations were carried out in accordance with a 10 sample analysis of variance (ANOVA) statistical model. One thousand one hundred sixteen genes had measurable expression 2 standard deviations above background and 50% of spots for any given sample for all hybridizations were positive. Expression values of significantly varying genes were clustered and a list of 408 genes (P < 0.05) with a 1.5 or greater fold change in at least one time point were chosen for further analysis. Seventy eight changed in a time-dependent manner with laminin-10 treatment, 85 changed with laminin-5, and 13 showed changes with both treatments. The 408 genes that passed a paired t-test in at least one time-dependent category were further analyzed using Pathway Miner. One of the largest gene association networks involved signal transduction in the growth factor-MAP kinase pathways. EGFR was validated by real-time PCR and laminin-10 mediated cell adhesion activated EGFR in DU-145 cells. Both laminins appear to be important signal transducers in prostate cancer.

Original languageEnglish (US)
Pages (from-to)1381-1390
Number of pages10
JournalProstate
Volume66
Issue number13
DOIs
StatePublished - Sep 13 2006
Externally publishedYes

Keywords

  • Laminin-10
  • Laminin-5
  • Prostate carcinoma cells
  • Signal transduction
  • cDNA microarray

ASJC Scopus subject areas

  • Oncology
  • Urology

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