HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor

Mehdi Kabani; Catherine McLellan; Deborah A. Raynes; Vince Guerriero; Jeffrey L. Brodsky

doi:10.1016/S0014-5793(02)03570-6

HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor

Mehdi Kabani, Catherine McLellan, Deborah A. Raynes, Vince Guerriero, Jeffrey L. Brodsky

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

The yeast FES1 and SLS1 genes encode conserved nucleotide exchange factors that act on the cytoplasmic and endoplasmic reticulum luminal Hsp70s, Ssa1p and BiP, respectively. We report here that mammalian HspBP1 is homologous to Fes1p and that HspBP1 promotes nucleotide dissociation from both Ssa1p and mammalian Hsc70. In contrast, Fes1p inefficiently strips nucleotide from mammalian Hsc70, and unlike HspBP1 does not inhibit chaperone-mediated protein refolding in vitro. Together, our data indicate that HspBP1 is a member of this new class of nucleotide exchange factors that exhibit varying degrees of compartment and species specificity.

Original language	English (US)
Pages (from-to)	339-342
Number of pages	4
Journal	FEBS Letters
Volume	531
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(02)03570-6
State	Published - Nov 6 2002

Keywords

Fes1p
Hsc70
Hsp70
HspBP1
Nucleotide exchange
Sls1p

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(02)03570-6

Cite this

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title = "HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor",

abstract = "The yeast FES1 and SLS1 genes encode conserved nucleotide exchange factors that act on the cytoplasmic and endoplasmic reticulum luminal Hsp70s, Ssa1p and BiP, respectively. We report here that mammalian HspBP1 is homologous to Fes1p and that HspBP1 promotes nucleotide dissociation from both Ssa1p and mammalian Hsc70. In contrast, Fes1p inefficiently strips nucleotide from mammalian Hsc70, and unlike HspBP1 does not inhibit chaperone-mediated protein refolding in vitro. Together, our data indicate that HspBP1 is a member of this new class of nucleotide exchange factors that exhibit varying degrees of compartment and species specificity.",

keywords = "Fes1p, Hsc70, Hsp70, HspBP1, Nucleotide exchange, Sls1p",

author = "Mehdi Kabani and Catherine McLellan and Raynes, {Deborah A.} and Vince Guerriero and Brodsky, {Jeffrey L.}",

note = "Funding Information: This work was supported by Grant MCB-0110331 to J.L.B. from the National Science Foundation.",

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doi = "10.1016/S0014-5793(02)03570-6",

language = "English (US)",

volume = "531",

pages = "339--342",

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T1 - HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor

AU - Kabani, Mehdi

AU - McLellan, Catherine

AU - Raynes, Deborah A.

AU - Guerriero, Vince

AU - Brodsky, Jeffrey L.

N1 - Funding Information: This work was supported by Grant MCB-0110331 to J.L.B. from the National Science Foundation.

PY - 2002/11/6

Y1 - 2002/11/6

N2 - The yeast FES1 and SLS1 genes encode conserved nucleotide exchange factors that act on the cytoplasmic and endoplasmic reticulum luminal Hsp70s, Ssa1p and BiP, respectively. We report here that mammalian HspBP1 is homologous to Fes1p and that HspBP1 promotes nucleotide dissociation from both Ssa1p and mammalian Hsc70. In contrast, Fes1p inefficiently strips nucleotide from mammalian Hsc70, and unlike HspBP1 does not inhibit chaperone-mediated protein refolding in vitro. Together, our data indicate that HspBP1 is a member of this new class of nucleotide exchange factors that exhibit varying degrees of compartment and species specificity.

AB - The yeast FES1 and SLS1 genes encode conserved nucleotide exchange factors that act on the cytoplasmic and endoplasmic reticulum luminal Hsp70s, Ssa1p and BiP, respectively. We report here that mammalian HspBP1 is homologous to Fes1p and that HspBP1 promotes nucleotide dissociation from both Ssa1p and mammalian Hsc70. In contrast, Fes1p inefficiently strips nucleotide from mammalian Hsc70, and unlike HspBP1 does not inhibit chaperone-mediated protein refolding in vitro. Together, our data indicate that HspBP1 is a member of this new class of nucleotide exchange factors that exhibit varying degrees of compartment and species specificity.

KW - Fes1p

KW - Hsc70

KW - Hsp70

KW - HspBP1

KW - Nucleotide exchange

KW - Sls1p

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AN - SCOPUS:0037032470

SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor

Abstract

Keywords

ASJC Scopus subject areas

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