HRD1-mediated METTL14 degradation regulates m6A mRNA modification to suppress ER proteotoxic liver disease

Juncheng Wei, Bryan T. Harada, Dan Lu, Ruihua Ma, Beixue Gao, Yanan Xu, Elena Montauti, Nikita Mani, Shuvam M. Chaudhuri, Shana Gregory, Samuel E. Weinberg, Donna D. Zhang, Richard Green, Chuan He, Deyu Fang

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) lumen triggers an unfolded protein response (UPR) for stress adaptation, the failure of which induces cell apoptosis and tissue/organ damage. The molecular switches underlying how the UPR selects for stress adaptation over apoptosis remain unknown. Here, we discovered that accumulation of unfolded/misfolded proteins selectively induces N6-adenosine-methyltransferase-14 (METTL14) expression. METTL14 promotes C/EBP-homologous protein (CHOP) mRNA decay through its 3′ UTR N6-methyladenosine (m6A) to inhibit its downstream pro-apoptotic target gene expression. UPR induces METTL14 expression by competing against the HRD1-ER-associated degradation (ERAD) machinery to block METTL14 ubiquitination and degradation. Therefore, mice with liver-specific METTL14 deletion are highly susceptible to both acute pharmacological and alpha-1 antitrypsin (AAT) deficiency-induced ER proteotoxic stress and liver injury. Further hepatic CHOP deletion protects METTL14 knockout mice from ER-stress-induced liver damage. Our study reveals a crosstalk between ER stress and mRNA m6A modification pathways, termed the ERm6A pathway, for ER stress adaptation to proteotoxicity.

Original languageEnglish (US)
Pages (from-to)5052-5065.e6
JournalMolecular cell
Volume81
Issue number24
DOIs
StatePublished - Dec 16 2021

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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