TY - JOUR
T1 - Horses With Pasture Asthma Have Airway Remodeling That Is Characteristic of Human Asthma
AU - Ferrari, Claudenir R.
AU - Cooley, Jim
AU - Mujahid, Nisma
AU - Costa, Lais R.
AU - Wills, Robert W.
AU - Johnson, Melanie E.
AU - Swiderski, Cyprianna E.
N1 - Funding Information:
Financial support for this research and its publication was provided by the Office of Graduate Research and the Departments of Clinical Sciences and Population and Preventative Medicine, College of Veterinary Medicine, Mississippi State University.
Publisher Copyright:
© 2017, © The Author(s) 2017.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Severe equine asthma, formerly recurrent airway obstruction (RAO), is the horse counterpart of human asthma, affecting horses maintained indoors in continental climates. Equine pasture asthma, formerly summer pasture RAO, is clinically similar but affects grazing horses during hot, humid conditions in the southeastern United States and United Kingdom. To advance translational relevance of equine pasture asthma to human asthma, histologic features of airway remodeling in human asthma were scored in lung lobes from 15 pasture asthma-affected and 9 control horses of mixed breeds. All noncartilaginous airways were scored using a standardized grading rubric (0–3) in hematoxylin and eosin (HE) and Movat’s pentachrome-stained sections; 15 airways were chosen randomly from each lobe for analysis. Logistic regression identified disease, age, and lobe effects on probability of histologic outcomes. Airway smooth muscle (odds ratio [OR] = 2.5, P <.001), goblet cell hyperplasia/metaplasia (OR = 37.6, P <.0001), peribronchiolar elastic system fibers (OR = 4.2, P <.001), peribronchiolar fibrosis (OR = 3.8, P =.01), airway occlusion by mucus/inflammation (OR = 4.2, P =.04), and airway adventitial inflammation (OR = 3.0, P =.01) were significantly greater in diseased airways. A novel complex tissue disorganization, designated terminal bronchiolar remodeling, was overrepresented in diseased airways (OR = 3.7, P <.0001). Distribution of terminal bronchiolar remodeling corresponded to putative sites of air trapping in human asthma, at secondary pulmonary lobules. Age (>15 years) was an independent risk factor for increased peribronchiolar fibrosis, elastic system fibers, and terminal bronchiolar remodeling. Remodeling differed significantly between lung lobes, congruent with nonhomogeneous remodeling in human asthma. Equine pasture asthma recapitulates airway remodeling in human asthma in a manner not achieved in induced animal asthma models, endorsing its translational relevance for human asthma investigation.
AB - Severe equine asthma, formerly recurrent airway obstruction (RAO), is the horse counterpart of human asthma, affecting horses maintained indoors in continental climates. Equine pasture asthma, formerly summer pasture RAO, is clinically similar but affects grazing horses during hot, humid conditions in the southeastern United States and United Kingdom. To advance translational relevance of equine pasture asthma to human asthma, histologic features of airway remodeling in human asthma were scored in lung lobes from 15 pasture asthma-affected and 9 control horses of mixed breeds. All noncartilaginous airways were scored using a standardized grading rubric (0–3) in hematoxylin and eosin (HE) and Movat’s pentachrome-stained sections; 15 airways were chosen randomly from each lobe for analysis. Logistic regression identified disease, age, and lobe effects on probability of histologic outcomes. Airway smooth muscle (odds ratio [OR] = 2.5, P <.001), goblet cell hyperplasia/metaplasia (OR = 37.6, P <.0001), peribronchiolar elastic system fibers (OR = 4.2, P <.001), peribronchiolar fibrosis (OR = 3.8, P =.01), airway occlusion by mucus/inflammation (OR = 4.2, P =.04), and airway adventitial inflammation (OR = 3.0, P =.01) were significantly greater in diseased airways. A novel complex tissue disorganization, designated terminal bronchiolar remodeling, was overrepresented in diseased airways (OR = 3.7, P <.0001). Distribution of terminal bronchiolar remodeling corresponded to putative sites of air trapping in human asthma, at secondary pulmonary lobules. Age (>15 years) was an independent risk factor for increased peribronchiolar fibrosis, elastic system fibers, and terminal bronchiolar remodeling. Remodeling differed significantly between lung lobes, congruent with nonhomogeneous remodeling in human asthma. Equine pasture asthma recapitulates airway remodeling in human asthma in a manner not achieved in induced animal asthma models, endorsing its translational relevance for human asthma investigation.
KW - airway remodeling
KW - animal
KW - disease models
KW - equine asthma
KW - heaves
KW - horses
KW - lungs
KW - summer pasture-associated recurrent airway obstruction (SPARAO)
KW - terminal bronchiolar remodeling
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U2 - 10.1177/0300985817741729
DO - 10.1177/0300985817741729
M3 - Article
C2 - 29254472
AN - SCOPUS:85038829378
SN - 0300-9858
VL - 55
SP - 144
EP - 158
JO - Pathologia veterinaria
JF - Pathologia veterinaria
IS - 1
ER -