HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells

Nafees Ahmad; Roshni Mehta; David T. Harris

doi:10.1016/j.virol.2011.01.033

HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells

Nafees Ahmad
, Roshni Mehta
, David T. Harris

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3⁺ and CD4⁺ naive (CD45RA⁺) and memory (CD45RO⁺) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3⁺ and CD4⁺ CD45RA⁺ and CD45RO⁺ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA⁺ and CD45RO⁺ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA⁺ and CD45RO⁺ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env^-R⁺ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA⁺ and CD45RO⁺ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.

Original language	English (US)
Pages (from-to)	39-46
Number of pages	8
Journal	Virology
Volume	413
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2011.01.033
State	Published - Apr 25 2011

Keywords

CD4
CXCR4 and CCR5 expression on naive and memory T-lymphocytes
Cord and adult blood naive and memory T-lymphocytes
HIV-1 gene expression
HIV-1 replication in naive and memory T-lymphocytes

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2011.01.033

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title = "HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells",

abstract = "Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA+) and memory (CD45RO+) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO+ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env-R+ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.",

keywords = "CD4, CXCR4 and CCR5 expression on naive and memory T-lymphocytes, Cord and adult blood naive and memory T-lymphocytes, HIV-1 gene expression, HIV-1 replication in naive and memory T-lymphocytes",

author = "Nafees Ahmad and Roshni Mehta and Harris, \{David T.\}",

note = "Funding Information: The authors would like to thank N. Landau (The Salk Institute, La Jolla, CA) for providing pHIV-NL-E-R + Luc and pSV-A-MLV-env plasmids, the AIDS Reference and Reagent program (Germantown, MD) for providing HIV-1 isolates, the Arizona Research Laboratories (ARL) specifically the flow cytometry core facility (Tucson, AZ), the cord blood donors and the phlebotomy group at University Medical Center (Tucson, AZ). This work was supported by a grant to NA by Arizona Biomedical Research Commission ( 7002 ).",

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doi = "10.1016/j.virol.2011.01.033",

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AU - Ahmad, Nafees

AU - Mehta, Roshni

AU - Harris, David T.

N1 - Funding Information: The authors would like to thank N. Landau (The Salk Institute, La Jolla, CA) for providing pHIV-NL-E-R + Luc and pSV-A-MLV-env plasmids, the AIDS Reference and Reagent program (Germantown, MD) for providing HIV-1 isolates, the Arizona Research Laboratories (ARL) specifically the flow cytometry core facility (Tucson, AZ), the cord blood donors and the phlebotomy group at University Medical Center (Tucson, AZ). This work was supported by a grant to NA by Arizona Biomedical Research Commission ( 7002 ).

PY - 2011/4/25

Y1 - 2011/4/25

N2 - Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA+) and memory (CD45RO+) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO+ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env-R+ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.

AB - Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA+) and memory (CD45RO+) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO+ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env-R+ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.

KW - CD4

KW - CXCR4 and CCR5 expression on naive and memory T-lymphocytes

KW - Cord and adult blood naive and memory T-lymphocytes

KW - HIV-1 gene expression

KW - HIV-1 replication in naive and memory T-lymphocytes

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JF - Virology

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ER -

HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells

Abstract

Keywords

ASJC Scopus subject areas

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