TY - JOUR
T1 - Hippocalcin in the olfactory epithelium
T2 - A mediator of second messenger signaling
AU - Mamman, Alex
AU - Simpson, Jeanette P.
AU - Nighorn, Alan
AU - Imanishi, Yoshikazu
AU - Palczewski, Krzysztof
AU - Ronnett, Gabriele V.
AU - Moon, Cheil
N1 - Funding Information:
The authors wish to thank Drs. M. Kobayashi for his kind gift of hippocalcin antibody and Andrei Alekseev for pBB131 and his technical advice. The NINDS (GVR) and NIDCD (G.V.R., J.P.S., and C.M.) and Howard Hughes Medical Institute (A.M.) supported this work. This research was also supported by NIH Grant EY09339, a grant from Research to Prevent Blindness, Inc. (RPB) to the Departments of Ophthalmology at the University of Washington, and a grant from the E.K. Bishop Foundation. K.P. is the RPB Senior Investigator.
PY - 2004/10/1
Y1 - 2004/10/1
N2 - Intracellular Ca2+ plays an important role in a variety of second messenger cascades. The function of Ca2+ is mediated, in part, by Ca2+-binding proteins such as calmodulin, calretinin, calbindin, neurocalcin, recoverin, and visinin-like proteins (VILIPs). These proteins are highly expressed in rat olfactory receptor neurons (ORNs) and are localized to distinct intracellular regions. In the present study, we have identified another Ca2+-binding protein, hippocalcin, in the rat olfactory epithelium (OE). Olfactory/brain hippocalcin shows high sequence homology with hippocalcins expressed in mice and humans. Hippocalcin was predominantly localized to the olfactory cilia, the site of the initial events of olfactory signal transduction, and was found to regulate the activity of ciliary adenylate cyclases (ACs) and particulate guanylyl cyclases (GCs) in a Ca 2+-dependent manner. These data indicate that hippocalcin is expressed in rat ORNs, and is likely to regulate second messenger cascades in a Ca2+-dependent manner.
AB - Intracellular Ca2+ plays an important role in a variety of second messenger cascades. The function of Ca2+ is mediated, in part, by Ca2+-binding proteins such as calmodulin, calretinin, calbindin, neurocalcin, recoverin, and visinin-like proteins (VILIPs). These proteins are highly expressed in rat olfactory receptor neurons (ORNs) and are localized to distinct intracellular regions. In the present study, we have identified another Ca2+-binding protein, hippocalcin, in the rat olfactory epithelium (OE). Olfactory/brain hippocalcin shows high sequence homology with hippocalcins expressed in mice and humans. Hippocalcin was predominantly localized to the olfactory cilia, the site of the initial events of olfactory signal transduction, and was found to regulate the activity of ciliary adenylate cyclases (ACs) and particulate guanylyl cyclases (GCs) in a Ca 2+-dependent manner. These data indicate that hippocalcin is expressed in rat ORNs, and is likely to regulate second messenger cascades in a Ca2+-dependent manner.
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U2 - 10.1016/j.bbrc.2004.07.123
DO - 10.1016/j.bbrc.2004.07.123
M3 - Article
C2 - 15336960
AN - SCOPUS:4444313771
SN - 0006-291X
VL - 322
SP - 1131
EP - 1139
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -