Abstract
The accelerating pace of genomics analysis has necessitated the abbreviation of DNA sample preparation protocols. We have developed a size-exclusion-based system for the rapid isolation of plasmid DNA in a 96-well microplate format. This high-speed protocol employs a modified alkaline lysis method for the preparation of the bacterial lysate, followed by three short vacuum filtration steps. Unlike traditional bind/wash/elute methods, there is no need to use chaotropic salts or ethanol. The samples are recovered from the top side of the MultiScreen96® PLASMID plates. Starting with bacterial cell pellets, the entire procedure for purifying the plasmid DNA can be performed in 30 min with a multichannel pipettor. The high yields, reproducibility, and quality of the plasmids make this system a good choice for any cloning or DNA sequencing operation.
Original language | English (US) |
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Pages (from-to) | 626-631 |
Number of pages | 6 |
Journal | BioTechniques |
Volume | 32 |
Issue number | 3 |
DOIs | |
State | Published - 2002 |
Externally published | Yes |
ASJC Scopus subject areas
- Biotechnology
- General Biochemistry, Genetics and Molecular Biology