TY - JOUR
T1 - High-resolution proton NMR spectra of human arterial plaque
AU - Zajicek, Jaroslav
AU - Pearlman, Justin D.
AU - Merickel, Michael B.
AU - Ayers, Carlos R.
AU - Brookeman, James R.
AU - Brown, Michael F.
N1 - Funding Information:
We thank Jeffrey Ellena and Amir Salmon for their assistance. This research was supported by National Institutes of Health Grant BY03754, the Whitaker Foundation, and the Thomas F. Jefress and Kate Miller Jeffress Memorial Trust. J.D.P. is an NIH Research Fellow in Cardiology. M.F.B. is the recipient of a Research Career Development Award from the and is an Alfred P. Sloan Research Fellow.
PY - 1987/12/16
Y1 - 1987/12/16
N2 - Well-resolved proton (1H) NMR spectra of solid human arterial plaque can be acquired. Studies have been carried out of human fatty plaque obtained postmortem (ex vivo), the total lipids extracted from human atheroma, and a model mixture of cholesteryl esters whose lipid composition resembles that of human atheroma. In each case, well-resolved 1H NMR spectra were obtained at body temperature (37°C), with little or no underlying broad signal. Such sharp 1H NMR spectra are typical of isotropic fluids, whereas solid and liquid-crystalline materials give rise to much broader spectral lines. The results suggest the sharp 1H NMR spectra of human atheromatous lesions at body temperature are due largely to the presence of intracellular and extracellular droplets of cholesteryl esters in the isotropic liquid phase. These findings provide a necessary basis for use of 1H NMR techniques to image quantitatively the lipid constituents of human atheroma in vivo, and to study their chemical and physical properties.
AB - Well-resolved proton (1H) NMR spectra of solid human arterial plaque can be acquired. Studies have been carried out of human fatty plaque obtained postmortem (ex vivo), the total lipids extracted from human atheroma, and a model mixture of cholesteryl esters whose lipid composition resembles that of human atheroma. In each case, well-resolved 1H NMR spectra were obtained at body temperature (37°C), with little or no underlying broad signal. Such sharp 1H NMR spectra are typical of isotropic fluids, whereas solid and liquid-crystalline materials give rise to much broader spectral lines. The results suggest the sharp 1H NMR spectra of human atheromatous lesions at body temperature are due largely to the presence of intracellular and extracellular droplets of cholesteryl esters in the isotropic liquid phase. These findings provide a necessary basis for use of 1H NMR techniques to image quantitatively the lipid constituents of human atheroma in vivo, and to study their chemical and physical properties.
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U2 - 10.1016/0006-291X(87)90386-X
DO - 10.1016/0006-291X(87)90386-X
M3 - Article
C2 - 3426583
AN - SCOPUS:0023604762
SN - 0006-291X
VL - 149
SP - 437
EP - 442
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -