High-resolution mass spectrometry of small molecules bound to membrane proteins

Joseph Gault, Joseph A.C. Donlan, Idlir Liko, Jonathan T.S. Hopper, Kallol Gupta, Nicholas G. Housden, Weston B. Struwe, Michael T. Marty, Todd Mize, Cherine Bechara, Ya Zhu, Beili Wu, Colin Kleanthous, Mikhail Belov, Eugen Damoc, Alexander Makarov, Carol V. Robinson

Research output: Contribution to journalArticlepeer-review

151 Scopus citations


Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.

Original languageEnglish (US)
Pages (from-to)333-336
Number of pages4
JournalNature Methods
Issue number4
StatePublished - Mar 30 2016
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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