High-resolution mass spectrometry of small molecules bound to membrane proteins

Joseph Gault; Joseph A.C. Donlan; Idlir Liko; Jonathan T.S. Hopper; Kallol Gupta; Nicholas G. Housden; Weston B. Struwe; Michael T. Marty; Todd Mize; Cherine Bechara; Ya Zhu; Beili Wu; Colin Kleanthous; Mikhail Belov; Eugen Damoc; Alexander Makarov; Carol V. Robinson

doi:10.1038/nmeth.3771

High-resolution mass spectrometry of small molecules bound to membrane proteins

Joseph Gault, Joseph A.C. Donlan, Idlir Liko, Jonathan T.S. Hopper, Kallol Gupta, Nicholas G. Housden, Weston B. Struwe, Michael T. Marty, Todd Mize, Cherine Bechara, Ya Zhu, Beili Wu, Colin Kleanthous, Mikhail Belov, Eugen Damoc, Alexander Makarov, Carol V. Robinson

Research output: Contribution to journal › Article › peer-review

195 Scopus citations

Abstract

Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.

Original language	English (US)
Pages (from-to)	333-336
Number of pages	4
Journal	Nature Methods
Volume	13
Issue number	4
DOIs	https://doi.org/10.1038/nmeth.3771
State	Published - Mar 30 2016
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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Gault, J., Donlan, J. A. C., Liko, I., Hopper, J. T. S., Gupta, K., Housden, N. G., Struwe, W. B., Marty, M. T., Mize, T., Bechara, C., Zhu, Y., Wu, B., Kleanthous, C., Belov, M., Damoc, E., Makarov, A., & Robinson, C. V. (2016). High-resolution mass spectrometry of small molecules bound to membrane proteins. Nature Methods, 13(4), 333-336. https://doi.org/10.1038/nmeth.3771

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AU - Gault, Joseph

AU - Donlan, Joseph A.C.

AU - Liko, Idlir

AU - Hopper, Jonathan T.S.

AU - Gupta, Kallol

AU - Housden, Nicholas G.

AU - Struwe, Weston B.

AU - Marty, Michael T.

AU - Mize, Todd

AU - Bechara, Cherine

AU - Zhu, Ya

AU - Wu, Beili

AU - Kleanthous, Colin

AU - Belov, Mikhail

AU - Damoc, Eugen

AU - Makarov, Alexander

AU - Robinson, Carol V.

PY - 2016/3/30

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N2 - Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.

AB - Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.

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High-resolution mass spectrometry of small molecules bound to membrane proteins

Abstract

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