High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives

Thomas P. Davis; Charles W. Gehrke; Charles W. Gehrke; Thomas D. Cunningham; Kenneth C. Kuo; Klaus O. Gerhardt; Harold D. Johnson; Charles H. Williams

doi:10.1016/S0378-4347(00)81516-9

High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives

Thomas P. Davis, Charles W. Gehrke, Charles W. Gehrke, Thomas D. Cunningham, Kenneth C. Kuo, Klaus O. Gerhardt, Harold D. Johnson, Charles H. Williams

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

A remarkably sensitive, simple and selective reversed-phase high-performance liquid chromatographic (HPLC) method has been developed, allowing, for the first time, the direct measurement of histamine, norepinephrine, octopamine, normetanephrine, dopamine, serotonin and tyramine in a single sample of plasma (2 ml), tissue (0.2 g), or urine. The biogenic amines were modified by pre-column derivatization with o-phthalaldehyde which stabilizes the molecules, aids in extraction, and improves HPLC detection at the nanogram level. To minimize losses during the sampling procedure a careful collection procedure was designed.

Original language	English (US)
Pages (from-to)	293-310
Number of pages	18
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	162
Issue number	3
DOIs	https://doi.org/10.1016/S0378-4347(00)81516-9
State	Published - Mar 1 1979
Externally published	Yes

ASJC Scopus subject areas

General Chemistry

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Davis, T. P., Gehrke, C. W., Gehrke, C. W., Cunningham, T. D., Kuo, K. C., Gerhardt, K. O., Johnson, H. D., & Williams, C. H. (1979). High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives. Journal of Chromatography B: Biomedical Sciences and Applications, 162(3), 293-310. https://doi.org/10.1016/S0378-4347(00)81516-9

High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives. / Davis, Thomas P.; Gehrke, Charles W.; Gehrke, Charles W. et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 162, No. 3, 01.03.1979, p. 293-310.

Research output: Contribution to journal › Article › peer-review

Davis, TP, Gehrke, CW, Gehrke, CW, Cunningham, TD, Kuo, KC, Gerhardt, KO, Johnson, HD & Williams, CH 1979, 'High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 162, no. 3, pp. 293-310. https://doi.org/10.1016/S0378-4347(00)81516-9

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High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives

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