TY - JOUR
T1 - HGF/SF is present in normal adult skeletal muscle and is capable of activating satellite cells
AU - Tatsumi, Ryuichi
AU - Anderson, Judy E.
AU - Nevoret, Cedrine J.
AU - Halevy, Orna
AU - Allen, Ronald E.
N1 - Funding Information:
This work was supported by grants from the USDA NRICRGP (R.E.A.), BARD (R.E.A. and O.H.), the Muscular Dystrophy Association (J.E.A.), and the Arizona Agriculture Experiment Station (R.E.A.). The technical assistance of Dr. Lucinda Rankin, Dr. Connie Temm-Grove, Dr. Parker Antin, Glenn Rice, Susanne Torsheya, Cynthia Vargas, and Roy Simpson is greatly appreciated, and helpful discussions with Dr. Shannon Sheehan were invaluable.
PY - 1998/2/1
Y1 - 1998/2/1
N2 - We have shown that hepatocyte growth factor/scatter factor can stimulate activation and early division of adult satellite cells in culture, and that the action of hepatocyte growth factor/scatter factor is similar to the action of the unidentified satellite cell activator found in extracts of crushed muscle. We now provide new evidence that hepatocyte growth factor/scatter factor is present in uninjured adult rat skeletal muscle and that the activating factor in crushed muscle extract is hepatocyte growth factor/scatter factor. Immunoblots of crushed muscle extract demonstrate the presence of hepatocyte growth factor/scatter factor. Furthermore, crushed muscle extract stimulates the scattering of cultured MDCK cells. Immunolocalization studies with adult rat skeletal muscle show the presence of hepatocyte growth factor/scatter factor in the extracellular matrix surrounding muscle fibers; in addition, the receptor for hepatocyte growth factor/scatter factor, c-met, is localized to putative satellite cells. In muscle from mdx mice, hepatocyte growth factor/scatter factor and c-met are colocalized in activated satellite cells in regions of muscle repair. Moreover, the satellite cell-activating activity of crushed muscle extract is abolished by preincubation with anti-hepatocyte growth factor antibodies. Finally, direct injection of hepatocyte growth factor/scatter factor into uninjured tibialis anterior muscle of 12-month-old rats stimulated satellite cell activation. These experiments demonstrate that hepatocyte growth factor/scatter factor is present in muscle, can be released upon injury, and has the ability to activate quiescent satellite cells in vivo.
AB - We have shown that hepatocyte growth factor/scatter factor can stimulate activation and early division of adult satellite cells in culture, and that the action of hepatocyte growth factor/scatter factor is similar to the action of the unidentified satellite cell activator found in extracts of crushed muscle. We now provide new evidence that hepatocyte growth factor/scatter factor is present in uninjured adult rat skeletal muscle and that the activating factor in crushed muscle extract is hepatocyte growth factor/scatter factor. Immunoblots of crushed muscle extract demonstrate the presence of hepatocyte growth factor/scatter factor. Furthermore, crushed muscle extract stimulates the scattering of cultured MDCK cells. Immunolocalization studies with adult rat skeletal muscle show the presence of hepatocyte growth factor/scatter factor in the extracellular matrix surrounding muscle fibers; in addition, the receptor for hepatocyte growth factor/scatter factor, c-met, is localized to putative satellite cells. In muscle from mdx mice, hepatocyte growth factor/scatter factor and c-met are colocalized in activated satellite cells in regions of muscle repair. Moreover, the satellite cell-activating activity of crushed muscle extract is abolished by preincubation with anti-hepatocyte growth factor antibodies. Finally, direct injection of hepatocyte growth factor/scatter factor into uninjured tibialis anterior muscle of 12-month-old rats stimulated satellite cell activation. These experiments demonstrate that hepatocyte growth factor/scatter factor is present in muscle, can be released upon injury, and has the ability to activate quiescent satellite cells in vivo.
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U2 - 10.1006/dbio.1997.8803
DO - 10.1006/dbio.1997.8803
M3 - Article
C2 - 9473336
AN - SCOPUS:0032006325
SN - 0012-1606
VL - 194
SP - 114
EP - 128
JO - Developmental biology
JF - Developmental biology
IS - 1
ER -