Heterotopic and orthotopic tracheal transplantation in mice used as models to study the development of obliterative airway disease

Obliterative airway disease (OAD) is the major complication after lung transplantations that limits long term survival. To study the pathophysiology, treatment and prevention of OAD, different animal models of tracheal transplantation in rodents have been developed. Here, we use two established models of trachea transplantation, the heterotopic and orthotopic model and demonstrate their advantages and limitations. For the heterotopic model, the donor trachea is wrapped into the greater omentum of the recipient, whereas the donor trachea is anastomosed by end-to-end anastomosis in the orthotopic model. In both models, the development of obliterative lesions histological similar to clinical OAD has been demonstrated. This video shows how to perform both, the heterotopic as well as the orthotopic tracheal transplantation technique in mice, and compares the time course of OAD development in both models using histology.