TY - JOUR
T1 - Heterorhabditis sonorensis n. sp. (Nematoda
T2 - Heterorhabditidae), a natural pathogen of the seasonal cicada Diceroprocta ornea (Walker) (Homoptera: Cicadidae) in the Sonoran desert
AU - Stock, S. Patricia
AU - Rivera-Orduño, Benjamin
AU - Flores-Lara, Yolanda
N1 - Funding Information:
We thank Patricia Delgado and M. Idalia Sotelo (Univ. Sonora, Mexico) who collaborated in the soil sampling in Caborca, Sonora, Mexico. We are also grateful to A. Sanborn (Barry University, FL) for identification of cicada species and to K. Nguyen for providing IJs of H. mexicana. This study constituted partial fulfillment for B. Rivera-Orduño’s Masters degree. This research was funded in part by a University of Arizona Faculty Small Grant to S.P. Stock.
PY - 2009/3
Y1 - 2009/3
N2 - A new Heterorhabditis species was isolated from nymphal stages of the seasonal cicada Diceroprocta ornea (Walker) in an asparagus field in the state of Sonora, Mexico. Concomitantly, another isolate of the same nematode species was also collected from an oak woodland habitat in the Chiricahua mountain range in southeastern Arizona. Morphological and molecular studies together with cross-hybridization tests indicate these two isolates are conspecific and represent a new undescribed Heterorhabditis sp. This new species is distinguished from other species in this genus by a combination of several qualitative and quantitative morphological traits. Key diagnostic features include: presence of a pronounced post-anal swelling in the hermaphrodite; male with nine pairs of bursal rays, with pairs 4 and 7 bent outwards and one pair of papillae placed on the cloacal opening, value of D% (average: 79); infective juveniles with a well developed cuticular tooth, long tail (average: 105 μm) and values of D% (average: 90) and E% (average: 99). In addition to these diagnostic characters, cross-hybridization tests between the new species with H. bacteriophora and H. mexicana yielded no fertile progeny. Comparison of ITS rDNA sequences with other available sequences of described species depicted the two isolates as a new species. Phylogenetic analysis of these sequence data placed H. sonorensis n. sp. as a member of the indica-group.
AB - A new Heterorhabditis species was isolated from nymphal stages of the seasonal cicada Diceroprocta ornea (Walker) in an asparagus field in the state of Sonora, Mexico. Concomitantly, another isolate of the same nematode species was also collected from an oak woodland habitat in the Chiricahua mountain range in southeastern Arizona. Morphological and molecular studies together with cross-hybridization tests indicate these two isolates are conspecific and represent a new undescribed Heterorhabditis sp. This new species is distinguished from other species in this genus by a combination of several qualitative and quantitative morphological traits. Key diagnostic features include: presence of a pronounced post-anal swelling in the hermaphrodite; male with nine pairs of bursal rays, with pairs 4 and 7 bent outwards and one pair of papillae placed on the cloacal opening, value of D% (average: 79); infective juveniles with a well developed cuticular tooth, long tail (average: 105 μm) and values of D% (average: 90) and E% (average: 99). In addition to these diagnostic characters, cross-hybridization tests between the new species with H. bacteriophora and H. mexicana yielded no fertile progeny. Comparison of ITS rDNA sequences with other available sequences of described species depicted the two isolates as a new species. Phylogenetic analysis of these sequence data placed H. sonorensis n. sp. as a member of the indica-group.
KW - Arizona
KW - Heterorhabditis sonorensis n. sp. - cicada - asparagus - oak woodlands
KW - Sonora
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U2 - 10.1016/j.jip.2008.11.011
DO - 10.1016/j.jip.2008.11.011
M3 - Article
C2 - 19114047
AN - SCOPUS:62249127307
SN - 0022-2011
VL - 100
SP - 175
EP - 184
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
IS - 3
ER -