TY - JOUR
T1 - Heterogeneity in the Effect of Albumin and Other Resuscitation Fluids on Intracellular Oxygen Free Radical Production
AU - Simpkins, Cuthbert O.
AU - Little, Donnie
AU - Brenner, Andrew
AU - Hill, January A.
AU - Griswold, John A.
AU - Rhee, Peter M.
AU - Hauser, Carl J.
AU - Schiller, Henry J.
AU - White-Owen, Cathy L.
PY - 2004/3
Y1 - 2004/3
N2 - Background: We hypothesized that by studying a suspension of a single cell type in various resuscitation fluids, we could compare their effects on parameters associated with cell death. Methods: Jurkat cells were suspended in resuscitation fluids. Using flow cytometry, light scatter, phosphatidylserine translocation, propidium iodide uptake, and intracellular H2O 2 production were measured. Results: Buffer (pH, 7.4) and albumin added to Ringer's lactate inhibited the adverse changes, including intracellular oxygen free radical production. Oxygen free radical production was variable within the cell population and inhibited by albumin but not other colloids or crystalloids. This correlated well with the ability of albumin to enhance metabolic activity. A flavoprotein inhibitor blocked H2O 2 production, suggesting that mitochondria are the source of the H2O2 and the variability. Conclusion: Oxygen free radical inhibition by albumin could explain both its beneficial and its harmful effects.
AB - Background: We hypothesized that by studying a suspension of a single cell type in various resuscitation fluids, we could compare their effects on parameters associated with cell death. Methods: Jurkat cells were suspended in resuscitation fluids. Using flow cytometry, light scatter, phosphatidylserine translocation, propidium iodide uptake, and intracellular H2O 2 production were measured. Results: Buffer (pH, 7.4) and albumin added to Ringer's lactate inhibited the adverse changes, including intracellular oxygen free radical production. Oxygen free radical production was variable within the cell population and inhibited by albumin but not other colloids or crystalloids. This correlated well with the ability of albumin to enhance metabolic activity. A flavoprotein inhibitor blocked H2O 2 production, suggesting that mitochondria are the source of the H2O2 and the variability. Conclusion: Oxygen free radical inhibition by albumin could explain both its beneficial and its harmful effects.
KW - Albumin
KW - Jurkat cells
KW - Lymphocytes
KW - Oxygen free radicals
KW - Resuscitation fluids
UR - https://www.scopus.com/pages/publications/1942540648
UR - https://www.scopus.com/pages/publications/1942540648#tab=citedBy
U2 - 10.1097/01.TA.0000112933.83144.23
DO - 10.1097/01.TA.0000112933.83144.23
M3 - Article
C2 - 15128126
AN - SCOPUS:1942540648
SN - 0022-5282
VL - 56
SP - 548
EP - 559
JO - Journal of Trauma - Injury, Infection and Critical Care
JF - Journal of Trauma - Injury, Infection and Critical Care
IS - 3
ER -