Hemostatic factors in rabbit limb lymph: Relationship to mechanisms regulating extravascular coagulation

Dzung T. Le, Peter Borgs, Thomas W. Toneff, Marlys H. Witte, Samuel I. Rapaport

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51 Scopus citations


Mechanisms regulating extravascular coagulation in interstitial fluids of peripheral tissues are poorly understood, since measurements of hemostatic factors in these fluids are unavailable. Because lymph from a body region reflects the composition of its interstitial fluid, we measured hemostatic factors in limb lymph of rabbits both as activity and as antigen. Mean lymph- to-plasma activity ratios were the following: fibrinogen, 0.28; prothrombin, 0.26; factor X, 0.27; factor VII, 0.17; and factors V and VIII, 0.08. All lymph fibrinogen was clottable; fibrin degradation products were absent. Lymph von Willebrand factor antigen was <10% of plasma antigen and consisted primarily of lower molecular weight multimers. Mean lymph-to-plasma activity ratio for antithrombin was 0.38 and for tissue factor pathway inhibitor the ratio was 0.40. Low levels of antithrombin-factor Xa were measurable in lymph. The data are compatible with a basal factor VIIa-tissue factor- catalyzed extravascular activation of factor X that is prevented from progressing to generation of fibrin in limb interstitial fluid and lymph by low levels of factor VIII and factor V and by the inhibitory activity of antithrombin and tissue factor pathway inhibitor.

Original languageEnglish (US)
Pages (from-to)H769-H776
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number3 43-3
StatePublished - Mar 1998


  • Antithrombin
  • Factor V
  • Factor VIII
  • Tissue factor pathway inhibitor
  • von Willebrand factor

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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