Abstract
Hemodilution (HD) has been associated with hypercoagulability. It was hypothesized that HD with lactated Ringer's solution (LR) would result in hypercoagulability in rabbits. Sedated rabbits (n = 12) underwent HD with LR (40% estimated blood volume replaced with five volumes of LR) via ear vessels. Key procoagulants and anticoagulant activities were assessed prior to and 3 h after HD. Hemostatic function was assessed with the activated coagulation time and platelet-inhibited thrombelastography. Circulating tissue factor activity was much more diluted (-67.2% from baseline) than tissue factor pathway inhibitor (-45.2%) or antithrombin (-9.5%) activities after HD. HD significantly decreased factor VIII complex activity (-31.5%) more than protein C activity (-5.9%), and factor X activity (-29.2%) was more diluted than antithrombin activity. The activated coagulation time and thrombelastography demonstrated a significant decrease in hemostatic function after HD. Hemodilution with LR caused hypocoagulability in the rabbit A greater decrease in circulating procoagulant activity than anticoagulant activity appears to be the mechanism underlying HD-mediated decreases in hemostasis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 55-59 |
| Number of pages | 5 |
| Journal | Blood Coagulation and Fibrinolysis |
| Volume | 15 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2004 |
| Externally published | Yes |
Keywords
- Crystalloids
- Factor VII
- Factor VIII
- Factor X
- Hemodilution
- Thrombelastography
- Tissue factor
- Tissue factor pathway inhibitor
ASJC Scopus subject areas
- Hematology