Hematopoietic prostaglandin D2 synthase in the chicken Harderian gland

T. R. Scott; A. R. Messersmith; W. J. McCrary; J. L. Herlong; S. C. Burgess

doi:10.1016/j.vetimm.2005.06.008

Hematopoietic prostaglandin D₂ synthase in the chicken Harderian gland

T. R. Scott, A. R. Messersmith, W. J. McCrary, J. L. Herlong, S. C. Burgess

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The Harderian gland (HG), a sero-mucous secreting organ in the eye orbit, has long been recognized as immunologically important in chickens. During experimentation to characterize immune components of the gland, proteomics analysis revealed the presence of hematopoietic prostaglandin D synthase (H-PGDS). Extraction of total RNA followed by RT-PCR produced cDNA of 597 base pairs. DNA sequencing revealed nucleic acid and predicted amino acid sequences that were 99% aligned with the one published sequence for chicken H-PGDS of the spleen. Alignment with murine, rat, and human H-PGDS were 69, 69, and 66%, respectively. Ocular vaccination of chickens with a Newcastle Disease/Infectious Bronchitis vaccine (Mass.-Ark. Strain) induced an increase in H-PGDS expression determined by real-time PCR. Furthermore, immunohistochemistry of frozen HG sections showed positive stained cells for both H-PGDS and mast cell tryptase in the sub-epithelial cell layers of the HG ducts. Based on the potent vasoactive role of PGD₂, it appears that the chicken HG is a site of active mucosal immunity partially mediated by PGD₂ synthesized by H-PGDS in the gland.

Original language	English (US)
Pages (from-to)	295-306
Number of pages	12
Journal	Veterinary Immunology and Immunopathology
Volume	108
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetimm.2005.06.008
State	Published - Dec 15 2005
Externally published	Yes

Keywords

Class-Sigma GST
H-PGD synthase
Harderian gland
PGD
Prostaglandin

ASJC Scopus subject areas

Immunology
General Veterinary

Access to Document

10.1016/j.vetimm.2005.06.008

Cite this

@article{67f36882295b4a4aa0d1d191db616358,

title = "Hematopoietic prostaglandin D2 synthase in the chicken Harderian gland",

abstract = "The Harderian gland (HG), a sero-mucous secreting organ in the eye orbit, has long been recognized as immunologically important in chickens. During experimentation to characterize immune components of the gland, proteomics analysis revealed the presence of hematopoietic prostaglandin D synthase (H-PGDS). Extraction of total RNA followed by RT-PCR produced cDNA of 597 base pairs. DNA sequencing revealed nucleic acid and predicted amino acid sequences that were 99% aligned with the one published sequence for chicken H-PGDS of the spleen. Alignment with murine, rat, and human H-PGDS were 69, 69, and 66%, respectively. Ocular vaccination of chickens with a Newcastle Disease/Infectious Bronchitis vaccine (Mass.-Ark. Strain) induced an increase in H-PGDS expression determined by real-time PCR. Furthermore, immunohistochemistry of frozen HG sections showed positive stained cells for both H-PGDS and mast cell tryptase in the sub-epithelial cell layers of the HG ducts. Based on the potent vasoactive role of PGD2, it appears that the chicken HG is a site of active mucosal immunity partially mediated by PGD2 synthesized by H-PGDS in the gland.",

keywords = "Class-Sigma GST, H-PGD synthase, Harderian gland, PGD, Prostaglandin",

author = "Scott, {T. R.} and Messersmith, {A. R.} and McCrary, {W. J.} and Herlong, {J. L.} and Burgess, {S. C.}",

note = "Funding Information: The authors thank Marcy Owens, Rebecca Ackerman, Teresa Smith, and Andy Mount of Clemson University, and T. Pechan and L.A. Shack of Mississippi State University for their technical assistance. Christy Scott at ISE America, Newberry, SC, kindly provided the Newcastle Disease/Infectious Bronchitis vaccine. Technical Contribution No. 5010 of the Clemson University Experiment Station. This material is based upon work supported by the CSREES/USDA, under project number SC-1700183. ",

year = "2005",

month = dec,

day = "15",

doi = "10.1016/j.vetimm.2005.06.008",

language = "English (US)",

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pages = "295--306",

journal = "Veterinary Immunology and Immunopathology",

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AU - Scott, T. R.

AU - Messersmith, A. R.

AU - McCrary, W. J.

AU - Herlong, J. L.

AU - Burgess, S. C.

N1 - Funding Information: The authors thank Marcy Owens, Rebecca Ackerman, Teresa Smith, and Andy Mount of Clemson University, and T. Pechan and L.A. Shack of Mississippi State University for their technical assistance. Christy Scott at ISE America, Newberry, SC, kindly provided the Newcastle Disease/Infectious Bronchitis vaccine. Technical Contribution No. 5010 of the Clemson University Experiment Station. This material is based upon work supported by the CSREES/USDA, under project number SC-1700183.

PY - 2005/12/15

Y1 - 2005/12/15

N2 - The Harderian gland (HG), a sero-mucous secreting organ in the eye orbit, has long been recognized as immunologically important in chickens. During experimentation to characterize immune components of the gland, proteomics analysis revealed the presence of hematopoietic prostaglandin D synthase (H-PGDS). Extraction of total RNA followed by RT-PCR produced cDNA of 597 base pairs. DNA sequencing revealed nucleic acid and predicted amino acid sequences that were 99% aligned with the one published sequence for chicken H-PGDS of the spleen. Alignment with murine, rat, and human H-PGDS were 69, 69, and 66%, respectively. Ocular vaccination of chickens with a Newcastle Disease/Infectious Bronchitis vaccine (Mass.-Ark. Strain) induced an increase in H-PGDS expression determined by real-time PCR. Furthermore, immunohistochemistry of frozen HG sections showed positive stained cells for both H-PGDS and mast cell tryptase in the sub-epithelial cell layers of the HG ducts. Based on the potent vasoactive role of PGD2, it appears that the chicken HG is a site of active mucosal immunity partially mediated by PGD2 synthesized by H-PGDS in the gland.

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