TY - JOUR
T1 - Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Malt1 signaling in neuroinflammation
AU - Cho, Jonathan J.
AU - Xu, Zhiwei
AU - Parthasarathy, Upasana
AU - Drashansky, Theodore T.
AU - Helm, Eric Y.
AU - Zuniga, Ashley N.
AU - Lorentsen, Kyle J.
AU - Mansouri, Samira
AU - Cho, Joshua Y.
AU - Edelmann, Mariola J.
AU - Duong, Duc M.
AU - Gehring, Torben
AU - Seeholzer, Thomas
AU - Krappmann, Daniel
AU - Uddin, Mohammad N.
AU - Califano, Danielle
AU - Wang, Rejean L.
AU - Jin, Lei
AU - Li, Hongmin
AU - Lv, Dongwen
AU - Zhou, Daohong
AU - Zhou, Liang
AU - Avram, Dorina
N1 - Funding Information:
We thank Linda (Xiaoping) Luo for D.A. laboratory management and animal colony maintenance, Monica C. Moore and Kevin Luque-Sanchez for technical assistance, and Alexander J. Kwiatkowski for assistance with statistical analysis. We thank Dr. Carlos M. de Noronha for the HA-Ub vector. We thank Dr. Vishva Dixit (Genentech, Inc.) and Dr. Robert C. Rickert for the Malt1–/− mice. National Multiple Sclerosis Society RG 4988A, NIH NIAID R01 AI133623, R01 AI067846, and UF Cancer Center supported the funding to D.A. The United States Navy HPSP funds medical education of J.J.C, T32AI007110 to K.J.L. and A.Z., T32DK074367 to T.T.D., R01DK105562 to L.Z., R21AI111045 to H.L. D.K. was funded by the DFG within CRC 1054 project A04.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Polyubiquitination promotes proteasomal degradation, or signaling and localization, of targeted proteins. Here we show that the E3 ubiquitin ligase Hectd3 is necessary for pathogenic Th17 cell generation in experimental autoimmune encephalomyelitis (EAE), a mouse model for human multiple sclerosis. Hectd3-deficient mice have lower EAE severity, reduced Th17 program and inefficient Th17 cell differentiation. However, Stat3, but not RORγt, has decreased polyubiquitination, as well as diminished tyrosine-705 activating phosphorylation. Additionally, non-degradative polyubiquitination of Malt1, critical for NF-κB activation and Th17 cell function, is reduced. Mechanistically, Hectd3 promotes K27-linked and K29-linked polyubiquitin chains on Malt1, and K27-linked polyubiquitin chains on Stat3. Moreover, Stat3 K180 and Malt1 K648 are targeted by Hectd3 for non-degradative polyubiquitination to mediate robust generation of RORγt + IL-17A hi effector CD4 + T cells. Thus, our studies delineate a mechanism connecting signaling related polyubiquitination of Malt1 and Stat3, leading to NF-kB activation and RORγt expression, to pathogenic Th17 cell function in EAE.
AB - Polyubiquitination promotes proteasomal degradation, or signaling and localization, of targeted proteins. Here we show that the E3 ubiquitin ligase Hectd3 is necessary for pathogenic Th17 cell generation in experimental autoimmune encephalomyelitis (EAE), a mouse model for human multiple sclerosis. Hectd3-deficient mice have lower EAE severity, reduced Th17 program and inefficient Th17 cell differentiation. However, Stat3, but not RORγt, has decreased polyubiquitination, as well as diminished tyrosine-705 activating phosphorylation. Additionally, non-degradative polyubiquitination of Malt1, critical for NF-κB activation and Th17 cell function, is reduced. Mechanistically, Hectd3 promotes K27-linked and K29-linked polyubiquitin chains on Malt1, and K27-linked polyubiquitin chains on Stat3. Moreover, Stat3 K180 and Malt1 K648 are targeted by Hectd3 for non-degradative polyubiquitination to mediate robust generation of RORγt + IL-17A hi effector CD4 + T cells. Thus, our studies delineate a mechanism connecting signaling related polyubiquitination of Malt1 and Stat3, leading to NF-kB activation and RORγt expression, to pathogenic Th17 cell function in EAE.
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U2 - 10.1038/s41467-019-08605-3
DO - 10.1038/s41467-019-08605-3
M3 - Article
C2 - 30741923
AN - SCOPUS:85061242751
VL - 10
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 701
ER -