TY - JOUR
T1 - Healing enhancement of diabetic wounds by locally infiltrated epidermal growth factor is associated with systemic oxidative stress reduction
AU - Ojalvo, Ariana García
AU - Acosta, Jorge Berlanga
AU - Marí, Yssel Mendoza
AU - Mayola, Maday Fernández
AU - Pérez, Calixto Valdés
AU - Gutiérrez, William Savigne
AU - Marichal, Ileydis Iglesias
AU - Seijas, Eduardo Álvarez
AU - Kautzman, Alicia Molina
AU - Pacheco, Angélica Estrada
AU - Armstrong, David G.
N1 - Publisher Copyright:
© 2016 Medicalhelplines.com Inc and John Wiley & Sons Ltd
PY - 2017/2/1
Y1 - 2017/2/1
N2 - The diabetic foot ulcer (DFU) is the leading cause of lower extremity amputation worldwide and is directly associated with comorbidity, disability and mortality. Oxidative stress mechanisms have been implicated in the pathogenesis of these wounds. Intra-lesional infiltration of epidermal growth factor has emerged as a potential therapeutic alternative to allow for physiological benefit while avoiding the proteolytic environment at the centre of the wound. The aim of this study was to characterise the response of patients with DFUs to epidermal growth factor treatment in terms of redox status markers. Experimental groups included patients with DFUs before and 3–4 weeks after starting treatment with epidermal growth factor; compensated and non-compensated diabetic patients without ulcers; and age-matched non-diabetic subjects. Evaluations comprised serum levels of oxidative stress and antioxidant reserve markers. Patients with DFUs exhibited the most disheveled biochemical profile, with elevated oxidative stress and low antioxidant reserves, with respect to non-ulcerated diabetic patients and to non-diabetic subjects. Epidermal growth factor intra-lesional administration was associated with a significant recovery of oxidative stress and antioxidant reserve markers. Altogether, our results indicate that epidermal growth factor intra-ulcer therapy contributes to restore systemic redox balance in patients with DFUs.
AB - The diabetic foot ulcer (DFU) is the leading cause of lower extremity amputation worldwide and is directly associated with comorbidity, disability and mortality. Oxidative stress mechanisms have been implicated in the pathogenesis of these wounds. Intra-lesional infiltration of epidermal growth factor has emerged as a potential therapeutic alternative to allow for physiological benefit while avoiding the proteolytic environment at the centre of the wound. The aim of this study was to characterise the response of patients with DFUs to epidermal growth factor treatment in terms of redox status markers. Experimental groups included patients with DFUs before and 3–4 weeks after starting treatment with epidermal growth factor; compensated and non-compensated diabetic patients without ulcers; and age-matched non-diabetic subjects. Evaluations comprised serum levels of oxidative stress and antioxidant reserve markers. Patients with DFUs exhibited the most disheveled biochemical profile, with elevated oxidative stress and low antioxidant reserves, with respect to non-ulcerated diabetic patients and to non-diabetic subjects. Epidermal growth factor intra-lesional administration was associated with a significant recovery of oxidative stress and antioxidant reserve markers. Altogether, our results indicate that epidermal growth factor intra-ulcer therapy contributes to restore systemic redox balance in patients with DFUs.
KW - Diabetes
KW - Diabetes complications
KW - Diabetic foot ulcers
KW - Epidermal growth factor
KW - Oxidative stress
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U2 - 10.1111/iwj.12592
DO - 10.1111/iwj.12592
M3 - Article
C2 - 27002919
AN - SCOPUS:84961801339
SN - 1742-4801
VL - 14
SP - 214
EP - 225
JO - International Wound Journal
JF - International Wound Journal
IS - 1
ER -