Guidelines for authors and reviewers on antibody use in physiology studies

Heddwen L. Brooks, Merry L. Lindsey

Research output: Contribution to journalReview articlepeer-review

57 Scopus citations


Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches.

Original languageEnglish (US)
Pages (from-to)H724-H732
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number4
StatePublished - Apr 2018


  • Antibody
  • Immunoblot analysis
  • Immunoblotting
  • Immunohistochemistry
  • Rigor and reproducibility

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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