Guidelines for authors and reviewers on antibody use in physiology studies

Heddwen L. Brooks; Merry L. Lindsey

doi:10.1152/ajpheart.00512.2017

Guidelines for authors and reviewers on antibody use in physiology studies

Heddwen L. Brooks, Merry L. Lindsey

Research output: Contribution to journal › Review article › peer-review

65 Scopus citations

Abstract

Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches.

Original language	English (US)
Pages (from-to)	H724-H732
Journal	American Journal of Physiology - Heart and Circulatory Physiology
Volume	314
Issue number	4
DOIs	https://doi.org/10.1152/ajpheart.00512.2017
State	Published - Apr 2018

Keywords

Antibody
Immunoblot analysis
Immunoblotting
Immunohistochemistry
Rigor and reproducibility

ASJC Scopus subject areas

Physiology
Cardiology and Cardiovascular Medicine
Physiology (medical)

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10.1152/ajpheart.00512.2017

Cite this

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title = "Guidelines for authors and reviewers on antibody use in physiology studies",

abstract = "Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches.",

keywords = "Antibody, Immunoblot analysis, Immunoblotting, Immunohistochemistry, Rigor and reproducibility",

author = "Brooks, {Heddwen L.} and Lindsey, {Merry L.}",

note = "Funding Information: We acknowledge support from the following funding agencies: National Institutes of Health Grants HL-131834, HL-075360, HL-129823, HL-051971, GM-104357, GM-115428, and GM-114833, Biomedical Laboratory Research and Development Service of the Veterans Affairs Office of Research and Development Grant 5I01BX000505, and the Sarver Heart Center, University of Arizona. Publisher Copyright: {\textcopyright} 2018 American Physiological Society. All rights reserved.",

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doi = "10.1152/ajpheart.00512.2017",

language = "English (US)",

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KW - Immunohistochemistry

KW - Rigor and reproducibility

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Guidelines for authors and reviewers on antibody use in physiology studies

Abstract

Keywords

ASJC Scopus subject areas

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