Growth of human rhinovirus in H1-HeLa cell suspension culture and purification of virions

Wai Ming Lee; Yin Chen; Wensheng Wang; Anne Mosser

doi:10.1007/978-1-4939-1571-2_5

Growth of human rhinovirus in H1-HeLa cell suspension culture and purification of virions

Wai Ming Lee, Yin Chen, Wensheng Wang, Anne Mosser

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

HeLa cell culture is the most widely used system for in vitro studies of the basic biology of human rhinovirus (HRV). It is also useful for making sufficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and making highly concentrated virus stock and highly purified virions. These purified HRV virions are free of cellular components and suitable for experiments that are sensitive to cellular contaminations.

Original language	English (US)
Pages (from-to)	49-61
Number of pages	13
Journal	Methods in Molecular Biology
Volume	1221
DOIs	https://doi.org/10.1007/978-1-4939-1571-2_5
State	Published - 2015

Keywords

HeLa cells
High-titer virus stock
Purified virions
Suspension culture

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-1571-2_5

Cite this

@article{01e24454b0de4ca6bcb298b2be7d67a0,

title = "Growth of human rhinovirus in H1-HeLa cell suspension culture and purification of virions",

abstract = "HeLa cell culture is the most widely used system for in vitro studies of the basic biology of human rhinovirus (HRV). It is also useful for making sufficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and making highly concentrated virus stock and highly purified virions. These purified HRV virions are free of cellular components and suitable for experiments that are sensitive to cellular contaminations.",

keywords = "HeLa cells, High-titer virus stock, Purified virions, Suspension culture",

author = "Lee, {Wai Ming} and Yin Chen and Wensheng Wang and Anne Mosser",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2015.",

year = "2015",

doi = "10.1007/978-1-4939-1571-2_5",

language = "English (US)",

volume = "1221",

pages = "49--61",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - Growth of human rhinovirus in H1-HeLa cell suspension culture and purification of virions

AU - Lee, Wai Ming

AU - Chen, Yin

AU - Wang, Wensheng

AU - Mosser, Anne

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - HeLa cell culture is the most widely used system for in vitro studies of the basic biology of human rhinovirus (HRV). It is also useful for making sufficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and making highly concentrated virus stock and highly purified virions. These purified HRV virions are free of cellular components and suitable for experiments that are sensitive to cellular contaminations.

AB - HeLa cell culture is the most widely used system for in vitro studies of the basic biology of human rhinovirus (HRV). It is also useful for making sufficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and making highly concentrated virus stock and highly purified virions. These purified HRV virions are free of cellular components and suitable for experiments that are sensitive to cellular contaminations.

KW - HeLa cells

KW - High-titer virus stock

KW - Purified virions

KW - Suspension culture

UR - http://www.scopus.com/inward/record.url?scp=84921775588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921775588&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-1571-2_5

DO - 10.1007/978-1-4939-1571-2_5

M3 - Article

C2 - 25261306

AN - SCOPUS:84921775588

SN - 1064-3745

VL - 1221

SP - 49

EP - 61

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Growth of human rhinovirus in H1-HeLa cell suspension culture and purification of virions

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this