TY - JOUR
T1 - Graft coronary artery disease in murine cardiac allografts
T2 - Proposal to meet the need for standardized assessment
AU - Tanaka, Masashi
AU - Fedoseyeva, Eugenia V.
AU - Robbins, Robert C.
N1 - Funding Information:
This study was supported in part by National Institutes of Health Grant HL65669.
PY - 2005/3
Y1 - 2005/3
N2 - Background: Inconsistency exists in assessing the severity of graft coronary artery disease (GCAD) in studies that use mouse models. The central issue associated with this inconsistency is the lack of a standardized approach for assessing mouse GCAD. Methods: We propose a new histologic definition of GCAD based on 3 successive stages (endotheliitis, premature lesion, and mature lesion) that mark the progression of this condition. In addition to these qualitative measures of GCAD, we propose including 2 additional morphometric parameters (percentage of luminal narrowing and intima-to-media ratio) and a measure of distribution (percentage of affected vessels) in the routine quantification of GCAD. Results: We introduce 2 new mouse models of GCAD as examples that satisfy these criteria. Conclusion: The proposed assessment criteria may simplify data collection and interpretation of results in various models of GCAD.
AB - Background: Inconsistency exists in assessing the severity of graft coronary artery disease (GCAD) in studies that use mouse models. The central issue associated with this inconsistency is the lack of a standardized approach for assessing mouse GCAD. Methods: We propose a new histologic definition of GCAD based on 3 successive stages (endotheliitis, premature lesion, and mature lesion) that mark the progression of this condition. In addition to these qualitative measures of GCAD, we propose including 2 additional morphometric parameters (percentage of luminal narrowing and intima-to-media ratio) and a measure of distribution (percentage of affected vessels) in the routine quantification of GCAD. Results: We introduce 2 new mouse models of GCAD as examples that satisfy these criteria. Conclusion: The proposed assessment criteria may simplify data collection and interpretation of results in various models of GCAD.
UR - http://www.scopus.com/inward/record.url?scp=14644425234&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14644425234&partnerID=8YFLogxK
U2 - 10.1016/j.healun.2003.12.011
DO - 10.1016/j.healun.2003.12.011
M3 - Article
C2 - 15737759
AN - SCOPUS:14644425234
SN - 1053-2498
VL - 24
SP - 316
EP - 322
JO - Journal of Heart and Lung Transplantation
JF - Journal of Heart and Lung Transplantation
IS - 3
ER -