Glucose-stimulated insulin secretion is not obligatorily linked to an increase in O2 consumption in βHC9 cells

Kleakchos K. Papas, Mary Ann C. Jarema

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


We investigated the effects of glucose on the rates of oxygen consumption (OCR) and insulin secretion (ISR) by βHC9 cells derived from mouse pancreatic islets with β-cell hyperplasia. Our results demonstrate that the OCR by βHC9 cells incubated in nutrient-rich DMEM is unaffected by glucose (0-25 mM), is dissociated from the ISR (which increases with the addition of glucose), and is always higher than that measured in PBS. Glucose (25 mM) increases both the OCR and ISR when added to nutrient-free PBS. On the basis of results presented here, we suggest that, contrary to the current consensus, the observed increases in the OCR by β-cells upon addition of glucose to nutrient-free buffers may be unrelated to the process of glucose- stimulated insulin secretion (GSIS) and, instead, related to nutrient starvation. We believe that a reevaluation of the implication of changes in OCR upon glucose stimulation in the process of GSIS is warranted and that OCR and ISR measurements should be performed in more physiological media to avoid nutrient starvation artifacts.

Original languageEnglish (US)
Pages (from-to)E1100-E1106
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number6 38-6
StatePublished - 1998


  • Bioartificial pancreas
  • Fuel hypothesis
  • Nutrient starvation
  • Tissue engineering

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)


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